An insect symbiotic virus promotes the transmission of a phytoarbovirus via inhibiting E3 ubiquitin ligase Sina

Co-infection with symbiotic viruses and arboviruses with synergistic effects in insect vectors are common in nature, but the underlying mechanism remains elusive. Here, we identify a novel symbiotic virus, leafhopper Recilia dorsalis bunyavirus (RdBV), which enhances the transmission efficiency of c...

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Veröffentlicht in:PLoS pathogens Jg. 21; H. 5; S. e1013178
Hauptverfasser: Wang, Hui, Zhang, Jieting, Liu, Runfa, Li, You, Du, Yu, Wei, Taiyun
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 01.05.2025
Public Library of Science (PLoS)
Schlagworte:
Animals
Antibodies
Arthropods
Bacteria
Biology and Life Sciences
Control
Degradation
Dengue fever
Disease transmission
Females
Genetic aspects
Genomes
Glycoproteins
Health aspects
Hemiptera - virology
Identification and classification
Infection
Infections
Insect Vectors - virology
Insects
Insects as carriers of disease
Ligases
Males
Medicine and Health Sciences
Microorganisms
Microscopy
Mosquitoes
Myc protein
Plant Diseases - virology
Plant virus diseases
Plant viruses
Prevention
Propagation
Proteins
Research and Analysis Methods
Rice
RNA polymerase
Symbiosis
Synergistic effect
Transmission efficiency
Ubiquitin
Ubiquitin-protein ligase
Ubiquitin-Protein Ligases - antagonists & inhibitors
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Ubiquitination
Vectors
Viroplasm
Virus Replication
Viruses
West Nile virus
China
Guangdong China
ISSN:1553-7374, 1553-7366, 1553-7374
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Zusammenfassung:Co-infection with symbiotic viruses and arboviruses with synergistic effects in insect vectors are common in nature, but the underlying mechanism remains elusive. Here, we identify a novel symbiotic virus, leafhopper Recilia dorsalis bunyavirus (RdBV), which enhances the transmission efficiency of cytorhabdovirus rice stripe mosaic virus (RSMV, a plant rhabdovirus) in field. RSMV infection activates the expression of R. dorsalis E3 ubiquitin ligase Seven in absentia (RdSina), while RdBV infection suppresses its expression. We show that RdSina directly targets and mediates the degradation of RSMV phosphoprotein (P), thereby attenuating the formation of P-induced viroplasm that are crucial for viral replication. RdSina interacts with nonstructural protein NSs2 of RdBV but does not mediate its ubiquitination. However, NSs2 competes with RSMV P for binding to RdSina, thus neutralizing RdSina’s ability in mediating P degradation. Furthermore, we find that the MYC transcription factor binds to the promoter sequences of RdSina, activating its transcription. However, NSs2 also directly binds to the same promoter sequences of RdSina and competitively suppresses MYC-activated RdSina transcription. Together, NSs2 obstructs the function of RdSina in mediating P degradation, ultimately promoting RSMV propagation in co-infected vectors. These findings elucidate how insect symbiotic viruses negatively regulate E3 ubiquitin ligases to benefit arbovirus transmission by co-infected insect vectors, which potentially is a common phenomenon in nature.
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These authors contributed equally: Hui Wang, Jieting Zhang.
The authors have declared that no competing interests exist
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1013178