Engineered cells as biosensing systems in biomedical analysis

Over the past two decades there have been great advances in biotechnology, including use of nucleic acids, proteins, and whole cells to develop a variety of molecular analytical tools for diagnostic, screening, and pharmaceutical applications. Through manipulation of bacterial plasmids and genomes,...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry Vol. 402; no. 10; pp. 3147 - 3159
Main Authors: Raut, Nilesh, O’Connor, Gregory, Pasini, Patrizia, Daunert, Sylvia
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer-Verlag 01.04.2012
Springer
Subjects:
Analysis
Analytical Chemistry
Animals
Antibacterial agents
Assessments
Bacteria
Bacteria - genetics
Bacterial Physiological Phenomena
Biochemistry
Biomedical Research
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cost analysis
Detection
Food Science
Genes, Reporter
Genetic Engineering
Genomes
Humans
Laboratory Medicine
Mathematical analysis
Miniaturization
Monitoring/Environmental Analysis
Plasmids - genetics
Plasmids - metabolism
Proteins
Quorum Sensing
Review
Screening
Technology application
Hydroxylated polychlorinated biphenyls
Whole-cell sensing system
Quorum sensing molecules
Cytarabine
Antibiotics
Biomedical analysis
Mercury
ISSN:1618-2642, 1618-2650, 1618-2650
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Over the past two decades there have been great advances in biotechnology, including use of nucleic acids, proteins, and whole cells to develop a variety of molecular analytical tools for diagnostic, screening, and pharmaceutical applications. Through manipulation of bacterial plasmids and genomes, bacterial whole-cell sensing systems have been engineered that can serve as novel methods for analyte detection and characterization, and as more efficient and cost-effective alternatives to traditional analytical techniques. Bacterial cell-based sensing systems are typically sensitive, specific and selective, rapid, easy to use, low-cost, and amenable to multiplexing, high-throughput, and miniaturization for incorporation into portable devices. This critical review is intended to provide an overview of available bacterial whole-cell sensing systems for assessment of a variety of clinically relevant analytes. Specifically, we examine whole-cell sensing systems for detection of bacterial quorum sensing molecules, organic and inorganic toxic compounds, and drugs, and for screening of antibacterial compounds for identification of their mechanisms of action. Methods used in the design and development of whole-cell sensing systems are also reviewed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Review-3
ISSN:1618-2642
1618-2650
1618-2650
DOI:10.1007/s00216-012-5756-6