Effect of iron on growth and lipid accumulation in Chlorella vulgaris

The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media w...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology Vol. 99; no. 11; pp. 4717 - 4722
Main Authors: Liu, Zhi-Yuan, Wang, Guang-Ce, Zhou, Bai-Cheng
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01.07.2008
[New York, NY]: Elsevier Ltd
Elsevier Science
Subjects:
aquatic plant culture
biodiesel
Biological and medical sciences
biomass
cell growth
centrifugation
Chlorella vulgaris
Chlorella vulgaris - cytology
Chlorella vulgaris - drug effects
Chlorella vulgaris - growth & development
Chlorella vulgaris - metabolism
Culture Media
cytology
drug effects
economic feasibility
FeCl 3
Fluorescence
Fundamental and applied biological sciences. Psychology
growth & development
iron
Iron - pharmacology
Lipid
lipid content
Lipid Metabolism
Lipid Metabolism - drug effects
lipids
metabolism
Microalgae
Nile Red
Nitrogen
Nitrogen - pharmacology
pharmacology
rapid methods
Spectrofluorimetry
Nile Red
FeCl 3
Microalgae
Spectrofluorimetry
Lipid
Fecl3
Algae
Chlorella vulgaris
Lipids
Iron III Chlorides
Chlorophyceae
Fluorescence spectrometry
Chlorophyta
Microorganism
ISSN:0960-8524, 1873-2976
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl 3 in the late growth phase increased the final cell density but did not induce lipid accumulation in cells. In experiment II, cells in the late-exponential growth phase were collected by centrifugation and re-inoculated into new media supplemented with five levels of Fe 3+ concentration. Total lipid content in cultures supplemented with 1.2 × 10 −5 mol L −1 FeCl 3 was up to 56.6% biomass by dry weight and was 3–7-fold that in other media supplemented with lower iron concentration. Moreover, a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed.
Bibliography:http://dx.doi.org/10.1016/j.biortech.2007.09.073
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2007.09.073