Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity

Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiat...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Nature communications Ročník 7; číslo 1; s. 11420
Hlavní autori: Chen, Yong, Buyel, Joschka J., Hanssen, Mark J. W., Siegel, Franziska, Pan, Ruping, Naumann, Jennifer, Schell, Michael, van der Lans, Anouk, Schlein, Christian, Froehlich, Holger, Heeren, Joerg, Virtanen, Kirsi A., van Marken Lichtenbelt, Wouter, Pfeifer, Alexander
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 27.04.2016
Nature Publishing Group
Nature Portfolio
Predmet:
13
13/106
13/109
13/44
38
38/90
631/337/384/331
631/443
692/308
692/53
Adipose tissue
Adipose Tissue, Brown - metabolism
Adult
Animals
Biomarkers
Body fat
Cohort Studies
Exosomes - metabolism
Female
Humanities and Social Sciences
Humans
Ionizing radiation
Kinases
Male
Mice, Inbred C57BL
MicroRNAs
MicroRNAs - blood
multidisciplinary
Science
Science (multidisciplinary)
Young Adult
Germany
Finland
ISSN:2041-1723, 2041-1723
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo . One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by 18 F-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans. Exosomes are RNA-containing lipid vesicles with roles in inter-tissue crosstalk. Here the authors show that exosome release from brown adipocytes is increased upon thermogenic activation, both in vitro and in vivo , and demonstrate that serum levels of exosomal miR-92 reflect brown fat activity in humans.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this work
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms11420