Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging

Tissues and organs are composed of distinct cell types that must operate in concert to perform physiological functions. Efforts to create high-dimensional biomarker catalogs of these cells have been largely based on single-cell sequencing approaches, which lack the spatial context required to unders...

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Veröffentlicht in:Nature methods Jg. 19; H. 3; S. 284 - 295
Hauptverfasser: Hickey, John W., Neumann, Elizabeth K., Radtke, Andrea J., Camarillo, Jeannie M., Beuschel, Rebecca T., Albanese, Alexandre, McDonough, Elizabeth, Hatler, Julia, Wiblin, Anne E., Fisher, Jeremy, Croteau, Josh, Small, Eliza C., Sood, Anup, Caprioli, Richard M., Angelo, R. Michael, Nolan, Garry P., Chung, Kwanghun, Hewitt, Stephen M., Germain, Ronald N., Spraggins, Jeffrey M., Lundberg, Emma, Snyder, Michael P., Kelleher, Neil L., Saka, Sinem K.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York Nature Publishing Group US 01.03.2022
Nature Publishing Group
Schlagworte:
631/1647/2067
631/1647/245/2225
706/648/697
Antibodies
antibody
Bioinformatics
Biological Microscopy
Biological Techniques
Biomarkers
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Cell Communication
Cell interactions
Cellular communication
Cellular structure
Diagnostic Imaging
Imaging
Life Sciences
Multiplexing
Organs
Peptide mapping
Perspective
Protein composition
Proteins
Proteomics
Robustness
ISSN:1548-7091, 1548-7105, 1548-7105
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Zusammenfassung:Tissues and organs are composed of distinct cell types that must operate in concert to perform physiological functions. Efforts to create high-dimensional biomarker catalogs of these cells have been largely based on single-cell sequencing approaches, which lack the spatial context required to understand critical cellular communication and correlated structural organization. To probe in situ biology with sufficient depth, several multiplexed protein imaging methods have been recently developed. Though these technologies differ in strategy and mode of immunolabeling and detection tags, they commonly utilize antibodies directed against protein biomarkers to provide detailed spatial and functional maps of complex tissues. As these promising antibody-based multiplexing approaches become more widely adopted, new frameworks and considerations are critical for training future users, generating molecular tools, validating antibody panels, and harmonizing datasets. In this Perspective, we provide essential resources, key considerations for obtaining robust and reproducible imaging data, and specialized knowledge from domain experts and technology developers. This Perspective offers guidance for robust and reproducible antibody-based highly multiplexed tissue imaging.
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J. W. H., E. K. N., A. J. R, J. M. C., and S. K. S. conceived the plan, designed figures, and wrote the manuscript. R. T. B. designed display items and helped write the manuscript. A. A., K. C., E. M., J. H., A. E. W., J. F., J. C., A. S., R. M. C., R. M. A., G. P. N., K. C., S. M. H., R. N. G., J. M. S., E. L., M. P. S., and N. L. K. provided domain expertise and assisted with the conception and writing of the manuscript.
Author contributions
ISSN:1548-7091
1548-7105
1548-7105
DOI:10.1038/s41592-021-01316-y