Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China

Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these...

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Bibliographic Details
Published in:Scientific reports Vol. 8; no. 1; pp. 10247 - 10
Main Authors: Huang, Keqiang, Zhang, Yong, Song, Yang, Cui, Hui, Yan, Dongmei, Zhu, Shuangli, Sun, Qiang, Tang, Haishu, Wang, Dongyan, Xu, Wenbo
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 06.07.2018
Nature Publishing Group
Nature Portfolio
Subjects:
13/106
45/23
45/29
45/90
631/208/325
692/308/174
Amino acid substitution
Amino acids
Antigenic characteristics
Cell culture
Disease control
Disease prevention
Epidemics
Epidemiology
Genomes
Genomic analysis
Genotype & phenotype
Humanities and Social Sciences
Laboratories
multidisciplinary
Nucleotide sequence
Paralysis
Phylogenetics
Recombination
Science
Science (multidisciplinary)
Serology
Temperature effects
Typing
Virulence
China
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4–89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P 3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3D pol region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-28469-9