Optimized and scalable synthesis of magnetic nanoparticles for RNA extraction in response to developing countries' needs in the detection and control of SARS-CoV-2

Ecuador is one of the most affected countries, with the coronavirus disease 2019 (COVID-19) infection, in Latin America derived from an ongoing economic crisis. One of the most important methods for COVID-19 detection is the use of techniques such as real time RT-PCR based on a previous extraction/p...

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Vydané v:Scientific reports Ročník 10; číslo 1; s. 19004
Hlavní autori: Chacón-Torres, Julio C., Reinoso, C., Navas-León, Daniela G., Briceño, Sarah, González, Gema
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 04.11.2020
Nature Publishing Group
Predmet:
639/925/357/354
639/925/357/551
Clinical Laboratory Techniques - methods
Coronavirus Infections - diagnosis
Coronaviruses
Costs and Cost Analysis
COVID-19
COVID-19 Testing
COVID-19 Vaccines
Developing Countries
Economic crisis
Humanities and Social Sciences
Humans
LDCs
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - economics
multidisciplinary
Nanoparticles
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - economics
Reverse Transcriptase Polymerase Chain Reaction - methods
Ribonucleic acid
RNA
RNA, Viral - chemistry
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
ISSN:2045-2322, 2045-2322
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Shrnutí:Ecuador is one of the most affected countries, with the coronavirus disease 2019 (COVID-19) infection, in Latin America derived from an ongoing economic crisis. One of the most important methods for COVID-19 detection is the use of techniques such as real time RT-PCR based on a previous extraction/purification of RNA procedure from nasopharyngeal cells using functionalized magnetic nanoparticles (MNP). This technique allows the processing of ~ 10,000 tests per day in private companies and around hundreds per day at local Universities guaranteeing to reach a wide range of the population. However, the main drawback of this method is the need for specialized MNP with a strong negative charge for the viral RNA extraction to detect the existence of the SARS-CoV-2 virus. Here we present a simplified low cost method to produce 10 g of nanoparticles in 100 mL of solution that was scaled to one litter by parallelizing the process 10 times in just two days and allowing for the possibility of making ~ 50,000 COVID-19 tests. This communication helps in reducing the cost of acquiring MNP for diverse biomolecular applications supporting developing country budgets constraints and chemical availability specially during the COVID-19 International Health Emergency.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-75798-9