Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp

The secreted mucilage trails of the diatom Navicula sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on th...

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Vydáno v:Scientific reports Ročník 9; číslo 1; s. 7342
Hlavní autoři: Chen, Lei, Weng, Ding, Du, Chuan, Wang, Jiadao, Cao, Shan
Médium: Journal Article
Jazyk:angličtina
Vydáno: London Nature Publishing Group UK 14.05.2019
Nature Publishing Group
Témata:
639/638/542/967
704/172/169/827
Adhesives - metabolism
Atomic force microscopy
Biofouling
Cell Adhesion
Cell Movement
Diatoms - cytology
Diatoms - metabolism
Humanities and Social Sciences
Locomotion
Mechanical properties
Microscopy
Motility
Mucilage
multidisciplinary
Navicula
Polymers
Polysaccharides
Polysaccharides - metabolism
Proteins - metabolism
Raman spectroscopy
Scanning electron microscopy
Science
Science (multidisciplinary)
Surface properties
Trails
ISSN:2045-2322, 2045-2322
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Shrnutí:The secreted mucilage trails of the diatom Navicula sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of in situ force mapping by AFM. The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak. Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom’s adhesion and locomotion. In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length. Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, —CH 2 — and —CH 3 asymmetric and symmetric stretches. This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-43663-z