Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis

Doris Wagner and colleagues define Polycomb response elements (PREs) that direct the placement of Polycomb repressive complex 2 (PRC2) at developmental genes in Arabidopsis . They identify transcription factor families that bind to PREs, physically interact with and recruit PRC2, and are required fo...

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Bibliographic Details
Published in:Nature genetics Vol. 49; no. 10; pp. 1546 - 1552
Main Authors: Xiao, Jun, Jin, Run, Yu, Xiang, Shen, Max, Wagner, John D, Pai, Armaan, Song, Claire, Zhuang, Michael, Klasfeld, Samantha, He, Chongsheng, Santos, Alexandre M, Helliwell, Chris, Pruneda-Paz, Jose L, Kay, Steve A, Lin, Xiaowei, Cui, Sujuan, Garcia, Meilin Fernandez, Clarenz, Oliver, Goodrich, Justin, Zhang, Xiaoyu, Austin, Ryan S, Bonasio, Roberto, Wagner, Doris
Format: Journal Article
Language:English
Published: New York Springer New York 01.10.2017
Nature Publishing Group
Subjects:
38/22
38/90
42/35
42/89
45
45/111
45/15
631/208/177
631/208/200
631/449/1659
Agriculture
Amino Acid Motifs
Animal Genetics and Genomics
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - biosynthesis
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
Arabidopsis thaliana
Binding Sites
Biomedicine
Cancer Research
Chromatin
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
DNA, Plant - metabolism
Epigenetic inheritance
Epigenetic Repression - genetics
Epigenetics
Flowers & plants
Flowers - growth & development
Fragments
Gene expression
Gene Expression Regulation, Plant
Gene Function
Gene Ontology
Gene Silencing
Genetic research
Genomes
High-Throughput Screening Assays
Human Genetics
Insects
letter
Multigene Family
Nucleotide sequence
Plant Leaves - ultrastructure
Plants, Genetically Modified
Polycomb group proteins
Polycomb Repressive Complex 2 - physiology
Polycomb-Group Proteins - physiology
Properties
Protein Binding
Protein Interaction Mapping
Proteins
Random variables
Recruitment
Regulatory sequences
Response Elements - genetics
Transcription factors
Transcription Factors - metabolism
ISSN:1061-4036, 1546-1718, 1546-1718
Online Access:Get full text
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Summary:Doris Wagner and colleagues define Polycomb response elements (PREs) that direct the placement of Polycomb repressive complex 2 (PRC2) at developmental genes in Arabidopsis . They identify transcription factor families that bind to PREs, physically interact with and recruit PRC2, and are required for gene silencing in vivo . Disruption of gene silencing by Polycomb protein complexes leads to homeotic transformations and altered developmental-phase identity in plants 1 , 2 , 3 , 4 , 5 . Here we define short genomic fragments, known as Polycomb response elements (PREs), that direct Polycomb repressive complex 2 (PRC2) placement at developmental genes regulated by silencing in Arabidopsis thaliana . We identify transcription factor families that bind to these PREs, colocalize with PRC2 on chromatin, physically interact with and recruit PRC2, and are required for PRC2-mediated gene silencing in vivo . Two of the cis sequence motifs enriched in the PREs are cognate binding sites for the identified transcription factors and are necessary and sufficient for PRE activity. Thus PRC2 recruitment in Arabidopsis relies in large part on binding of trans -acting factors to cis -localized DNA sequence motifs.
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ISSN:1061-4036
1546-1718
1546-1718
DOI:10.1038/ng.3937