Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis

Doris Wagner and colleagues define Polycomb response elements (PREs) that direct the placement of Polycomb repressive complex 2 (PRC2) at developmental genes in Arabidopsis . They identify transcription factor families that bind to PREs, physically interact with and recruit PRC2, and are required fo...

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Vydáno v:	Nature genetics Ročník 49; číslo 10; s. 1546 - 1552
Hlavní autoři:	Xiao, Jun, Jin, Run, Yu, Xiang, Shen, Max, Wagner, John D, Pai, Armaan, Song, Claire, Zhuang, Michael, Klasfeld, Samantha, He, Chongsheng, Santos, Alexandre M, Helliwell, Chris, Pruneda-Paz, Jose L, Kay, Steve A, Lin, Xiaowei, Cui, Sujuan, Garcia, Meilin Fernandez, Clarenz, Oliver, Goodrich, Justin, Zhang, Xiaoyu, Austin, Ryan S, Bonasio, Roberto, Wagner, Doris
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York Springer New York 01.10.2017 Nature Publishing Group
Témata:	38/22 38/90 42/35 42/89 45 45/111 45/15 631/208/177 631/208/200 631/449/1659 Agriculture Amino Acid Motifs Animal Genetics and Genomics Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - biosynthesis Arabidopsis Proteins - genetics Arabidopsis Proteins - physiology Arabidopsis thaliana Binding Sites Biomedicine Cancer Research Chromatin Deoxyribonucleic acid DNA DNA, Plant - genetics DNA, Plant - metabolism Epigenetic inheritance Epigenetic Repression - genetics Epigenetics Flowers & plants Flowers - growth & development Fragments Gene expression Gene Expression Regulation, Plant Gene Function Gene Ontology Gene Silencing Genetic research Genomes High-Throughput Screening Assays Human Genetics Insects letter Multigene Family Nucleotide sequence Plant Leaves - ultrastructure Plants, Genetically Modified Polycomb group proteins Polycomb Repressive Complex 2 - physiology Polycomb-Group Proteins - physiology Properties Protein Binding Protein Interaction Mapping Proteins Random variables Recruitment Regulatory sequences Response Elements - genetics Transcription factors Transcription Factors - metabolism
ISSN:	1061-4036, 1546-1718, 1546-1718
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Shrnutí:	Doris Wagner and colleagues define Polycomb response elements (PREs) that direct the placement of Polycomb repressive complex 2 (PRC2) at developmental genes in Arabidopsis . They identify transcription factor families that bind to PREs, physically interact with and recruit PRC2, and are required for gene silencing in vivo . Disruption of gene silencing by Polycomb protein complexes leads to homeotic transformations and altered developmental-phase identity in plants 1 , 2 , 3 , 4 , 5 . Here we define short genomic fragments, known as Polycomb response elements (PREs), that direct Polycomb repressive complex 2 (PRC2) placement at developmental genes regulated by silencing in Arabidopsis thaliana . We identify transcription factor families that bind to these PREs, colocalize with PRC2 on chromatin, physically interact with and recruit PRC2, and are required for PRC2-mediated gene silencing in vivo . Two of the cis sequence motifs enriched in the PREs are cognate binding sites for the identified transcription factors and are necessary and sufficient for PRE activity. Thus PRC2 recruitment in Arabidopsis relies in large part on binding of trans -acting factors to cis -localized DNA sequence motifs.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1061-4036 1546-1718 1546-1718
DOI:	10.1038/ng.3937