Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia

The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune f...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	eLife Ročník 10
Hlavní autoři:	Schuurman, Alex R, Reijnders, Tom DY, Saris, Anno, Ramirez Moral, Ivan, Schinkel, Michiel, de Brabander, Justin, van Linge, Christine, Vermeulen, Louis, Scicluna, Brendon P, Wiersinga, W Joost, Vieira Braga, Felipe A, van der Poll, Tom
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England eLife Science Publications, Ltd 23.08.2021 eLife Sciences Publications Ltd eLife Sciences Publications, Ltd
Témata:	Adult Aged Analysis Antibodies Biological response modifiers CD8 antigen Cell cycle Community-Acquired Infections - immunology Coronaviruses COVID-19 COVID-19 - immunology Cytotoxicity Ethylenediaminetetraacetic acid Female Genomics Health aspects Humans Immune response Immunology and Inflammation Infections Influenza Influenza A Influenza, Human - immunology Interferon Killer cells Leukocytes (mononuclear) Leukocytes, Mononuclear - immunology Male Microbiology and Infectious Disease Middle Aged Monocytes Natural killer cells PBMC Peripheral blood mononuclear cells Pneumonia Pneumonia - immunology Pneumonia - virology Proteomics Proteomics - methods SARS-CoV-2 - immunology Severe acute respiratory syndrome coronavirus 2 Single-Cell Analysis Tagging Transcription Transcriptome Transcriptomics Canada Netherlands COVID-19 Pneumonia infectious disease inflammation microbiology PBMC human immunology
ISSN:	2050-084X, 2050-084X
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors also contributed equally to this work.
ISSN:	2050-084X 2050-084X
DOI:	10.7554/eLife.69661