Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia

The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune f...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:eLife Jg. 10
Hauptverfasser: Schuurman, Alex R, Reijnders, Tom DY, Saris, Anno, Ramirez Moral, Ivan, Schinkel, Michiel, de Brabander, Justin, van Linge, Christine, Vermeulen, Louis, Scicluna, Brendon P, Wiersinga, W Joost, Vieira Braga, Felipe A, van der Poll, Tom
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England eLife Science Publications, Ltd 23.08.2021
eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
Schlagworte:
Adult
Aged
Analysis
Antibodies
Biological response modifiers
CD8 antigen
Cell cycle
Community-Acquired Infections - immunology
Coronaviruses
COVID-19
COVID-19 - immunology
Cytotoxicity
Ethylenediaminetetraacetic acid
Female
Genomics
Health aspects
Humans
Immune response
Immunology and Inflammation
Infections
Influenza
Influenza A
Influenza, Human - immunology
Interferon
Killer cells
Leukocytes (mononuclear)
Leukocytes, Mononuclear - immunology
Male
Microbiology and Infectious Disease
Middle Aged
Monocytes
Natural killer cells
PBMC
Peripheral blood mononuclear cells
Pneumonia
Pneumonia - immunology
Pneumonia - virology
Proteomics
Proteomics - methods
SARS-CoV-2 - immunology
Severe acute respiratory syndrome coronavirus 2
Single-Cell Analysis
Tagging
Transcription
Transcriptome
Transcriptomics
Canada
Netherlands
COVID-19
Pneumonia
infectious disease
inflammation
microbiology
PBMC
human
immunology
ISSN:2050-084X, 2050-084X
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors also contributed equally to this work.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.69661