A genetically encoded sensor for measuring serotonin dynamics
Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, hi...
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| Vydáno v: | Nature neuroscience Ročník 24; číslo 5; s. 746 - 752 |
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| Hlavní autoři: | , , , , , , , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
New York
Nature Publishing Group US
01.05.2021
Nature Publishing Group |
| Témata: | |
| ISSN: | 1097-6256, 1546-1726, 1546-1726 |
| On-line přístup: | Získat plný text |
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| Abstract | Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB
5-HT
) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB
5-HT
detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.
The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. |
|---|---|
| AbstractList | Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB
) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB
detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB 5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB.sub.5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB.sub.5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB.sub.5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB.sub.5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB5-HT) sensor with high sensitivity, selectivity, subsecond kinetics, and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice, and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. |
| Audience | Academic |
| Author | Pan, Sunlei Zhang, Peng Feng, Jiesi Shin, Mimi Song, Kun Xu, Min Wan, Jinxia Venton, B. Jill Zhang, Yajun Zou, Jing Deng, Fei Hao, Suyu Zeng, Jianzhi Li, Yulong Zhu, J. Julius Jing, Miao Qian, Tongrui Li, Xuelin Peng, Wanling |
| AuthorAffiliation | 4 Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China 7 Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA 8 Chinese Institute for Brain Research, Beijing 102206, China 3 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China 6 Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA 90089, USA 5 Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA 1 State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China 2 PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China |
| AuthorAffiliation_xml | – name: 8 Chinese Institute for Brain Research, Beijing 102206, China – name: 2 PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China – name: 4 Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China – name: 6 Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA 90089, USA – name: 3 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China – name: 1 State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China – name: 5 Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA – name: 7 Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA |
| Author_xml | – sequence: 1 givenname: Jinxia orcidid: 0000-0001-5942-1465 surname: Wan fullname: Wan, Jinxia organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 2 givenname: Wanling surname: Peng fullname: Peng, Wanling organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 3 givenname: Xuelin surname: Li fullname: Li, Xuelin organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 4 givenname: Tongrui orcidid: 0000-0002-0329-4687 surname: Qian fullname: Qian, Tongrui organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 5 givenname: Kun orcidid: 0000-0002-6379-2805 surname: Song fullname: Song, Kun organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 6 givenname: Jianzhi orcidid: 0000-0002-5380-6281 surname: Zeng fullname: Zeng, Jianzhi organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 7 givenname: Fei surname: Deng fullname: Deng, Fei organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 8 givenname: Suyu surname: Hao fullname: Hao, Suyu organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 9 givenname: Jiesi surname: Feng fullname: Feng, Jiesi organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 10 givenname: Peng surname: Zhang fullname: Zhang, Peng organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 11 givenname: Yajun surname: Zhang fullname: Zhang, Yajun organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 12 givenname: Jing surname: Zou fullname: Zou, Jing organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Department of Biological Sciences, Neurobiology Section, University of Southern California – sequence: 13 givenname: Sunlei surname: Pan fullname: Pan, Sunlei organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 14 givenname: Mimi surname: Shin fullname: Shin, Mimi organization: Department of Chemistry, University of Virginia – sequence: 15 givenname: B. Jill surname: Venton fullname: Venton, B. Jill organization: Department of Chemistry, University of Virginia – sequence: 16 givenname: J. Julius orcidid: 0000-0002-1879-983X surname: Zhu fullname: Zhu, J. Julius organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 17 givenname: Miao surname: Jing fullname: Jing, Miao organization: Chinese Institute for Brain Research – sequence: 18 givenname: Min orcidid: 0000-0002-6936-7543 surname: Xu fullname: Xu, Min organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 19 givenname: Yulong orcidid: 0000-0002-9166-9919 surname: Li fullname: Li, Yulong email: yulongli@pku.edu.cn organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33821000$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/srep20889 10.1073/pnas.1019483108 10.7554/eLife.12727 10.1046/j.1471-4159.1998.70031077.x 10.1038/nmeth.1318 10.3389/fncir.2020.00025 10.1016/S0092-8674(00)00183-5 10.1016/S0306-4522(97)00438-7 10.1016/S0893-133X(00)00126-3 10.1126/science.aat4422 10.1016/j.neuron.2019.02.037 10.2174/157015911795017254 10.1038/nbt1172 10.1007/s12195-012-0232-0 10.1016/j.cell.2018.07.043 10.1074/jbc.RA118.001975 10.1016/j.cell.2005.09.037 10.1038/s41598-017-07820-6 10.1016/0304-3940(88)90691-X 10.1016/j.neuron.2019.05.038 10.1073/pnas.1204520109 10.1016/j.cell.2018.06.042 10.3389/fpsyg.2015.01805 10.1016/S0278-5846(02)00338-X 10.1038/nature10424 10.1021/acs.analchem.8b02114 10.1038/nn.4143 10.1126/science.274.5292.1527 10.1073/pnas.0710487105 10.1016/j.neuron.2004.10.006 10.1016/j.str.2008.10.008 10.1038/ncomms10503 10.1038/nbt.4184 10.7554/eLife.49424 10.1016/0024-3205(94)00876-0 10.1016/j.cell.2018.01.001 10.1038/nsmb.3014 10.1016/S1043-9471(05)80049-7 10.1073/pnas.89.5.1817 |
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| Copyright | The Author(s), under exclusive licence to Springer Nature America, Inc. 2021 COPYRIGHT 2021 Nature Publishing Group The Author(s), under exclusive licence to Springer Nature America, Inc. 2021. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Y.L. conceived and supervised the project. J.W., M.J., F.D., S.H., J.F., J. Zou, and S.P. performed the experiments related to developing, optimizing, and characterizing the sensor in cultured HEK293T cells and neurons. T.Q. performed the experiments using AAVs in slices. P.Z., Y.Z. and M.S. performed the simultaneous imaging and FSCV experiments using Sindbis virus in slices under the supervision of J.J.Z and B.J.V. X.L. and J. Zeng performed the Drosophila experiments. W.P. and K.S. performed the fiber-photometry recordings in behaving mice under the supervision of M.X. J.W. performed the two-photon imaging in head-fixed mice. All authors contributed to the data interpretation and analysis. Y.L. and J.W. wrote the manuscript with input from all other authors. Author contributions |
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| References | Portas (CR3) 1998; 83 Peng (CR14) 2018; 172 CR15 Bajar (CR12) 2016; 6 Kroeze (CR33) 2015; 22 Yu (CR23) 2005; 123 Yusa (CR37) 2011; 478 Sun (CR10) 2018; 174 Dana (CR35) 2016; 5 Feng (CR11) 2019; 102 Gibson (CR36) 2009; 6 Bunin, Prioleau, Mailman, Wightman (CR6) 1998; 70 Lesch (CR1) 1996; 274 Lee (CR21) 2011; 108 Harada (CR16) 2017; 7 Vaswani, Linda, Ramesh (CR4) 2003; 27 Ishimura (CR19) 1988; 91 Oikonomou (CR26) 2019; 103 Patriarchi (CR9) 2018; 360 Wan (CR17) 2018; 293 Keene (CR22) 2004; 44 Li (CR2) 2016; 7 Barnea (CR18) 2008; 105 Cantu (CR39) 2020; 14 Xu (CR24) 2015; 18 CR28 Pfeiffer, Truman, Rubin (CR34) 2012; 109 Hagino (CR31) 2011; 9 Ren (CR27) 2019; 8 Wang, Shui, Kotlikoff, Sondermann (CR32) 2008; 16 Ren (CR25) 2018; 175 Fuller (CR5) 1994; 55 Pedelacq, Cabantous, Tran, Terwilliger, Waldo (CR13) 2006; 24 Candelario, Chachisvilis (CR7) 2012; 5 Jing (CR8) 2018; 36 Shin, Venton (CR38) 2018; 90 Waddell, Armstrong, Kitamoto, Kaiser, Quinn (CR20) 2000; 103 Bicks, Koike, Akbarian, Morishita (CR29) 2015; 6 Liechti, Saur, Gamma, Hell, Vollenweider (CR30) 2000; 23 AC Keene (823_CR22) 2004; 44 J Candelario (823_CR7) 2012; 5 823_CR15 BT Bajar (823_CR12) 2016; 6 J Feng (823_CR11) 2019; 102 BD Pfeiffer (823_CR34) 2012; 109 H Dana (823_CR35) 2016; 5 M Shin (823_CR38) 2018; 90 M Xu (823_CR24) 2015; 18 CM Portas (823_CR3) 1998; 83 K Ishimura (823_CR19) 1988; 91 Y Hagino (823_CR31) 2011; 9 F Sun (823_CR10) 2018; 174 D Yu (823_CR23) 2005; 123 Q Wan (823_CR17) 2018; 293 J Ren (823_CR25) 2018; 175 DG Gibson (823_CR36) 2009; 6 KP Lesch (823_CR1) 1996; 274 MA Bunin (823_CR6) 1998; 70 Q Wang (823_CR32) 2008; 16 K Yusa (823_CR37) 2011; 478 823_CR28 J Ren (823_CR27) 2019; 8 Y Li (823_CR2) 2016; 7 JD Pedelacq (823_CR13) 2006; 24 S Waddell (823_CR20) 2000; 103 G Oikonomou (823_CR26) 2019; 103 ME Liechti (823_CR30) 2000; 23 M Jing (823_CR8) 2018; 36 WK Kroeze (823_CR33) 2015; 22 G Barnea (823_CR18) 2008; 105 LK Bicks (823_CR29) 2015; 6 RW Fuller (823_CR5) 1994; 55 Y Peng (823_CR14) 2018; 172 K Harada (823_CR16) 2017; 7 DA Cantu (823_CR39) 2020; 14 M Vaswani (823_CR4) 2003; 27 T Patriarchi (823_CR9) 2018; 360 PT Lee (823_CR21) 2011; 108 |
| References_xml | – volume: 6 year: 2016 ident: CR12 article-title: Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting publication-title: Sci. Rep. doi: 10.1038/srep20889 – volume: 108 start-page: 13794 year: 2011 end-page: 13799 ident: CR21 article-title: Serotonin–mushroom body circuit modulating the formation of anesthesia-resistant memory in publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1019483108 – volume: 5 start-page: e12727 year: 2016 ident: CR35 article-title: Sensitive red protein calcium indicators for imaging neural activity publication-title: eLife doi: 10.7554/eLife.12727 – volume: 70 start-page: 1077 year: 1998 end-page: 1087 ident: CR6 article-title: Release and uptake rates of 5-hydroxytryptamine in the dorsal raphe and substantia nigra reticulata of the rat brain publication-title: J. Neurochem. doi: 10.1046/j.1471-4159.1998.70031077.x – volume: 6 start-page: 343 year: 2009 end-page: 345 ident: CR36 article-title: Enzymatic assembly of DNA molecules up to several hundred kilobases publication-title: Nat. Methods doi: 10.1038/nmeth.1318 – volume: 14 start-page: 25 year: 2020 ident: CR39 article-title: EZcalcium: open-source toolbox for analysis of calcium imaging data publication-title: Front. Neural Circuits doi: 10.3389/fncir.2020.00025 – volume: 103 start-page: 805 year: 2000 end-page: 813 ident: CR20 article-title: The amnesiac gene product is expressed in two neurons in the brain that are critical for memory publication-title: Cell doi: 10.1016/S0092-8674(00)00183-5 – volume: 83 start-page: 807 year: 1998 end-page: 814 ident: CR3 article-title: On-line detection of extracellular levels of serotonin in dorsal raphe nucleus and frontal cortex over the sleep/wake cycle in the freely moving rat publication-title: Neuroscience doi: 10.1016/S0306-4522(97)00438-7 – volume: 23 start-page: 396 year: 2000 end-page: 404 ident: CR30 article-title: Psychological and physiological effects of MDMA (‘ecstasy’) after pretreatment with the 5-HT2 antagonist ketanserin in healthy humans publication-title: Neuropsychopharmacology doi: 10.1016/S0893-133X(00)00126-3 – volume: 360 start-page: eaat4422 year: 2018 ident: CR9 article-title: Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors publication-title: Science doi: 10.1126/science.aat4422 – volume: 102 start-page: 745 year: 2019 end-page: 761 ident: CR11 article-title: A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine publication-title: Neuron doi: 10.1016/j.neuron.2019.02.037 – volume: 9 start-page: 91 year: 2011 end-page: 95 ident: CR31 article-title: Effects of MDMA on extracellular dopamine and serotonin levels in mice lacking dopamine and/or serotonin transporters publication-title: Curr. Neuropharmacol. doi: 10.2174/157015911795017254 – volume: 24 start-page: 79 year: 2006 end-page: 88 ident: CR13 article-title: Engineering and characterization of a superfolder green fluorescent protein publication-title: Nat. Biotechnol. doi: 10.1038/nbt1172 – volume: 5 start-page: 277 year: 2012 end-page: 286 ident: CR7 article-title: Mechanical stress stimulates conformational changes in 5-hydroxytryptamine receptor 1B in bone cells publication-title: Cell. Mol. Bioeng. doi: 10.1007/s12195-012-0232-0 – volume: 175 start-page: 472 year: 2018 end-page: 487 ident: CR25 article-title: Anatomically defined and functionally distinct dorsal raphe serotonin sub-systems publication-title: Cell doi: 10.1016/j.cell.2018.07.043 – volume: 293 start-page: 7466 year: 2018 end-page: 7473 ident: CR17 article-title: Mini G protein probes for active G protein-coupled receptors (GPCRs) in live cells publication-title: J. Biol. Chem. doi: 10.1074/jbc.RA118.001975 – volume: 123 start-page: 945 year: 2005 end-page: 957 ident: CR23 article-title: DPM neurons form a delayed and branch-specific memory trace after olfactory classical conditioning publication-title: Cell doi: 10.1016/j.cell.2005.09.037 – volume: 7 year: 2017 ident: CR16 article-title: Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging publication-title: Sci. Rep. doi: 10.1038/s41598-017-07820-6 – volume: 91 start-page: 265 year: 1988 end-page: 270 ident: CR19 article-title: Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain publication-title: Neurosci. Lett. doi: 10.1016/0304-3940(88)90691-X – volume: 103 start-page: 686 year: 2019 end-page: 701 ident: CR26 article-title: The serotonergic raphe promote sleep in zebrafish and mice publication-title: Neuron doi: 10.1016/j.neuron.2019.05.038 – volume: 109 start-page: 6626 year: 2012 end-page: 6631 ident: CR34 article-title: Using translational enhancers to increase transgene expression in publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1204520109 – volume: 174 start-page: 481 year: 2018 end-page: 496 ident: CR10 article-title: A genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice publication-title: Cell doi: 10.1016/j.cell.2018.06.042 – volume: 6 start-page: 1805 year: 2015 ident: CR29 article-title: Prefrontal cortex and social cognition in mouse and man publication-title: Front. Psychol. doi: 10.3389/fpsyg.2015.01805 – volume: 27 start-page: 85 year: 2003 end-page: 102 ident: CR4 article-title: Role of selective serotonin reuptake inhibitors in psychiatric disorders: a comprehensive review publication-title: Prog. Neuropsychopharmacol. Biol. Psychiatry doi: 10.1016/S0278-5846(02)00338-X – ident: CR15 – volume: 478 start-page: 391 year: 2011 end-page: 394 ident: CR37 article-title: Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells publication-title: Nature doi: 10.1038/nature10424 – volume: 90 start-page: 10318 year: 2018 end-page: 10325 ident: CR38 article-title: Electrochemical measurements of acetylcholine-stimulated dopamine release in adult brains publication-title: Anal. Chem. doi: 10.1021/acs.analchem.8b02114 – volume: 18 start-page: 1641 year: 2015 end-page: 1647 ident: CR24 article-title: Basal forebrain circuit for sleep–wake control publication-title: Nat. Neurosci. doi: 10.1038/nn.4143 – volume: 274 start-page: 1527 year: 1996 end-page: 1531 ident: CR1 article-title: Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region publication-title: Science doi: 10.1126/science.274.5292.1527 – volume: 105 start-page: 64 year: 2008 end-page: 69 ident: CR18 article-title: The genetic design of signaling cascades to record receptor activation publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.0710487105 – volume: 44 start-page: 521 year: 2004 end-page: 533 ident: CR22 article-title: Diverse odor-conditioned memories require uniquely timed dorsal paired medial neuron output publication-title: Neuron doi: 10.1016/j.neuron.2004.10.006 – volume: 16 start-page: 1817 year: 2008 end-page: 1827 ident: CR32 article-title: Structural basis for calcium sensing by GCaMP2 publication-title: Structure doi: 10.1016/j.str.2008.10.008 – volume: 7 year: 2016 ident: CR2 article-title: Serotonin neurons in the dorsal raphe nucleus encode reward signals publication-title: Nat. Commun. doi: 10.1038/ncomms10503 – volume: 36 start-page: 726 year: 2018 end-page: 737 ident: CR8 article-title: A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies publication-title: Nat. Biotechnol. doi: 10.1038/nbt.4184 – volume: 8 start-page: e49424 year: 2019 ident: CR27 article-title: Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei publication-title: eLife doi: 10.7554/eLife.49424 – ident: CR28 – volume: 55 start-page: 163 year: 1994 end-page: 167 ident: CR5 article-title: Uptake inhibitors increase extracellular serotonin concentration measured by brain microdialysis publication-title: Life Sci. doi: 10.1016/0024-3205(94)00876-0 – volume: 172 start-page: 719 year: 2018 end-page: 730 ident: CR14 article-title: 5-HT2C receptor structures reveal the structural basis of GPCR polypharmacology publication-title: Cell doi: 10.1016/j.cell.2018.01.001 – volume: 22 start-page: 362 year: 2015 end-page: 369 ident: CR33 article-title: PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome publication-title: Nat. Struct. Mol. Biol. doi: 10.1038/nsmb.3014 – volume: 274 start-page: 1527 year: 1996 ident: 823_CR1 publication-title: Science doi: 10.1126/science.274.5292.1527 – volume: 103 start-page: 805 year: 2000 ident: 823_CR20 publication-title: Cell doi: 10.1016/S0092-8674(00)00183-5 – volume: 478 start-page: 391 year: 2011 ident: 823_CR37 publication-title: Nature doi: 10.1038/nature10424 – volume: 23 start-page: 396 year: 2000 ident: 823_CR30 publication-title: Neuropsychopharmacology doi: 10.1016/S0893-133X(00)00126-3 – volume: 44 start-page: 521 year: 2004 ident: 823_CR22 publication-title: Neuron doi: 10.1016/j.neuron.2004.10.006 – volume: 90 start-page: 10318 year: 2018 ident: 823_CR38 publication-title: Anal. Chem. doi: 10.1021/acs.analchem.8b02114 – volume: 27 start-page: 85 year: 2003 ident: 823_CR4 publication-title: Prog. Neuropsychopharmacol. Biol. Psychiatry doi: 10.1016/S0278-5846(02)00338-X – volume: 24 start-page: 79 year: 2006 ident: 823_CR13 publication-title: Nat. Biotechnol. doi: 10.1038/nbt1172 – volume: 123 start-page: 945 year: 2005 ident: 823_CR23 publication-title: Cell doi: 10.1016/j.cell.2005.09.037 – volume: 6 year: 2016 ident: 823_CR12 publication-title: Sci. Rep. doi: 10.1038/srep20889 – volume: 172 start-page: 719 year: 2018 ident: 823_CR14 publication-title: Cell doi: 10.1016/j.cell.2018.01.001 – ident: 823_CR15 doi: 10.1016/S1043-9471(05)80049-7 – volume: 5 start-page: e12727 year: 2016 ident: 823_CR35 publication-title: eLife doi: 10.7554/eLife.12727 – volume: 293 start-page: 7466 year: 2018 ident: 823_CR17 publication-title: J. Biol. Chem. doi: 10.1074/jbc.RA118.001975 – volume: 18 start-page: 1641 year: 2015 ident: 823_CR24 publication-title: Nat. Neurosci. doi: 10.1038/nn.4143 – volume: 7 year: 2017 ident: 823_CR16 publication-title: Sci. Rep. doi: 10.1038/s41598-017-07820-6 – volume: 6 start-page: 343 year: 2009 ident: 823_CR36 publication-title: Nat. Methods doi: 10.1038/nmeth.1318 – volume: 5 start-page: 277 year: 2012 ident: 823_CR7 publication-title: Cell. Mol. Bioeng. doi: 10.1007/s12195-012-0232-0 – volume: 108 start-page: 13794 year: 2011 ident: 823_CR21 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1019483108 – volume: 7 year: 2016 ident: 823_CR2 publication-title: Nat. Commun. doi: 10.1038/ncomms10503 – volume: 55 start-page: 163 year: 1994 ident: 823_CR5 publication-title: Life Sci. doi: 10.1016/0024-3205(94)00876-0 – volume: 175 start-page: 472 year: 2018 ident: 823_CR25 publication-title: Cell doi: 10.1016/j.cell.2018.07.043 – volume: 6 start-page: 1805 year: 2015 ident: 823_CR29 publication-title: Front. Psychol. doi: 10.3389/fpsyg.2015.01805 – volume: 174 start-page: 481 year: 2018 ident: 823_CR10 publication-title: Cell doi: 10.1016/j.cell.2018.06.042 – volume: 105 start-page: 64 year: 2008 ident: 823_CR18 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.0710487105 – ident: 823_CR28 doi: 10.1073/pnas.89.5.1817 – volume: 102 start-page: 745 year: 2019 ident: 823_CR11 publication-title: Neuron doi: 10.1016/j.neuron.2019.02.037 – volume: 36 start-page: 726 year: 2018 ident: 823_CR8 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.4184 – volume: 83 start-page: 807 year: 1998 ident: 823_CR3 publication-title: Neuroscience doi: 10.1016/S0306-4522(97)00438-7 – volume: 70 start-page: 1077 year: 1998 ident: 823_CR6 publication-title: J. Neurochem. doi: 10.1046/j.1471-4159.1998.70031077.x – volume: 360 start-page: eaat4422 year: 2018 ident: 823_CR9 publication-title: Science doi: 10.1126/science.aat4422 – volume: 16 start-page: 1817 year: 2008 ident: 823_CR32 publication-title: Structure doi: 10.1016/j.str.2008.10.008 – volume: 8 start-page: e49424 year: 2019 ident: 823_CR27 publication-title: eLife doi: 10.7554/eLife.49424 – volume: 9 start-page: 91 year: 2011 ident: 823_CR31 publication-title: Curr. Neuropharmacol. doi: 10.2174/157015911795017254 – volume: 109 start-page: 6626 year: 2012 ident: 823_CR34 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1204520109 – volume: 91 start-page: 265 year: 1988 ident: 823_CR19 publication-title: Neurosci. Lett. doi: 10.1016/0304-3940(88)90691-X – volume: 22 start-page: 362 year: 2015 ident: 823_CR33 publication-title: Nat. Struct. Mol. Biol. doi: 10.1038/nsmb.3014 – volume: 14 start-page: 25 year: 2020 ident: 823_CR39 publication-title: Front. Neural Circuits doi: 10.3389/fncir.2020.00025 – volume: 103 start-page: 686 year: 2019 ident: 823_CR26 publication-title: Neuron doi: 10.1016/j.neuron.2019.05.038 |
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| Title | A genetically encoded sensor for measuring serotonin dynamics |
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