A genetically encoded sensor for measuring serotonin dynamics
Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, hi...
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| Vydáno v: | Nature neuroscience Ročník 24; číslo 5; s. 746 - 752 |
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| Hlavní autoři: | , , , , , , , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
New York
Nature Publishing Group US
01.05.2021
Nature Publishing Group |
| Témata: | |
| ISSN: | 1097-6256, 1546-1726, 1546-1726 |
| On-line přístup: | Získat plný text |
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| Abstract | Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB
5-HT
) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB
5-HT
detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.
The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. |
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| AbstractList | Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB
) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB
detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB 5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB.sub.5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB.sub.5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB.sub.5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB.sub.5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded GPCR-Activation-Based 5-HT (GRAB5-HT) sensor with high sensitivity, selectivity, subsecond kinetics, and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice, and provides new insights into the dynamics and mechanisms of 5-HT signaling. Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. |
| Audience | Academic |
| Author | Pan, Sunlei Zhang, Peng Feng, Jiesi Shin, Mimi Song, Kun Xu, Min Wan, Jinxia Venton, B. Jill Zhang, Yajun Zou, Jing Deng, Fei Hao, Suyu Zeng, Jianzhi Li, Yulong Zhu, J. Julius Jing, Miao Qian, Tongrui Li, Xuelin Peng, Wanling |
| AuthorAffiliation | 4 Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China 7 Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA 8 Chinese Institute for Brain Research, Beijing 102206, China 3 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China 6 Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA 90089, USA 5 Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA 1 State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China 2 PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China |
| AuthorAffiliation_xml | – name: 8 Chinese Institute for Brain Research, Beijing 102206, China – name: 2 PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China – name: 4 Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China – name: 6 Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA 90089, USA – name: 3 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China – name: 1 State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China – name: 5 Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA – name: 7 Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA |
| Author_xml | – sequence: 1 givenname: Jinxia orcidid: 0000-0001-5942-1465 surname: Wan fullname: Wan, Jinxia organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 2 givenname: Wanling surname: Peng fullname: Peng, Wanling organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 3 givenname: Xuelin surname: Li fullname: Li, Xuelin organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 4 givenname: Tongrui orcidid: 0000-0002-0329-4687 surname: Qian fullname: Qian, Tongrui organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 5 givenname: Kun orcidid: 0000-0002-6379-2805 surname: Song fullname: Song, Kun organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 6 givenname: Jianzhi orcidid: 0000-0002-5380-6281 surname: Zeng fullname: Zeng, Jianzhi organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 7 givenname: Fei surname: Deng fullname: Deng, Fei organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 8 givenname: Suyu surname: Hao fullname: Hao, Suyu organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research – sequence: 9 givenname: Jiesi surname: Feng fullname: Feng, Jiesi organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 10 givenname: Peng surname: Zhang fullname: Zhang, Peng organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 11 givenname: Yajun surname: Zhang fullname: Zhang, Yajun organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 12 givenname: Jing surname: Zou fullname: Zou, Jing organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Department of Biological Sciences, Neurobiology Section, University of Southern California – sequence: 13 givenname: Sunlei surname: Pan fullname: Pan, Sunlei organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University – sequence: 14 givenname: Mimi surname: Shin fullname: Shin, Mimi organization: Department of Chemistry, University of Virginia – sequence: 15 givenname: B. Jill surname: Venton fullname: Venton, B. Jill organization: Department of Chemistry, University of Virginia – sequence: 16 givenname: J. Julius orcidid: 0000-0002-1879-983X surname: Zhu fullname: Zhu, J. Julius organization: Department of Pharmacology, University of Virginia School of Medicine – sequence: 17 givenname: Miao surname: Jing fullname: Jing, Miao organization: Chinese Institute for Brain Research – sequence: 18 givenname: Min orcidid: 0000-0002-6936-7543 surname: Xu fullname: Xu, Min organization: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences – sequence: 19 givenname: Yulong orcidid: 0000-0002-9166-9919 surname: Li fullname: Li, Yulong email: yulongli@pku.edu.cn organization: State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33821000$$D View this record in MEDLINE/PubMed |
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| Copyright | The Author(s), under exclusive licence to Springer Nature America, Inc. 2021 COPYRIGHT 2021 Nature Publishing Group The Author(s), under exclusive licence to Springer Nature America, Inc. 2021. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Y.L. conceived and supervised the project. J.W., M.J., F.D., S.H., J.F., J. Zou, and S.P. performed the experiments related to developing, optimizing, and characterizing the sensor in cultured HEK293T cells and neurons. T.Q. performed the experiments using AAVs in slices. P.Z., Y.Z. and M.S. performed the simultaneous imaging and FSCV experiments using Sindbis virus in slices under the supervision of J.J.Z and B.J.V. X.L. and J. Zeng performed the Drosophila experiments. W.P. and K.S. performed the fiber-photometry recordings in behaving mice under the supervision of M.X. J.W. performed the two-photon imaging in head-fixed mice. All authors contributed to the data interpretation and analysis. Y.L. and J.W. wrote the manuscript with input from all other authors. Author contributions |
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| Title | A genetically encoded sensor for measuring serotonin dynamics |
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