Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient

High soil phosphorus (P) concentration is frequently shown to reduce root colonization by arbuscular mycorrhizal (AM) fungi, but the influence of P on the diversity of colonizing AM fungi is uncertain. We used terminal restriction fragment length polymorphism (T-RFLP) of 18S rDNA and cloning to asse...

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Bibliographic Details
Published in:The New phytologist Vol. 198; no. 2; pp. 546 - 556
Main Authors: Gosling, Paul, Mead, Andrew, Proctor, Maude, Hammond, John P., Bending, Gary D.
Format: Journal Article
Language:English
Published: England New Phytologist Trust 01.04.2013
Wiley Subscription Services, Inc
Blackwell Publishing Ltd
Subjects:
Agricultural soils
Agrology
Arbuscular mycorrhizas
Cloning
Cluster Analysis
Colonization
Colony Count, Microbial
Concentration gradient
Corn
diversity
DNA
DNA Restriction Enzymes - metabolism
Fungi
Glycine max - microbiology
Host plants
maize (Zea mays)
Mycorrhizae - drug effects
Mycorrhizae - growth & development
Mycorrhizae - physiology
mycorrhizas
Phosphorus
phosphorus (P)
Phosphorus - pharmacology
Plants
Plants - drug effects
Plants - microbiology
Polymorphism
Restriction fragment length polymorphism
Sampling
Soil
Soil - chemistry
Soil ecology
Soil fungi
Soil samples
Soils
soybean (Glycene max)
Soybeans
Time Factors
vesicular arbuscular mycorrhizae
Viola - microbiology
Viola arvensis
Viola arvensis (field violet)
Violas
Zea mays
Zea mays - microbiology
ISSN:0028-646X, 1469-8137, 1469-8137
Online Access:Get full text
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Summary:High soil phosphorus (P) concentration is frequently shown to reduce root colonization by arbuscular mycorrhizal (AM) fungi, but the influence of P on the diversity of colonizing AM fungi is uncertain. We used terminal restriction fragment length polymorphism (T-RFLP) of 18S rDNA and cloning to assess diversity of AM fungi colonizing maize (Zea mays), soybean (Glycene max) and field violet (Viola arvensis) at three time points in one season along a P gradient of 10–280 mg l−1 in the field. Percentage AM colonization changed between sampling time points but was not reduced by high soil P except in maize. There was no significant difference in AM diversity between sampling time points. Diversity was reduced at concentrations of P > 25 mg l−1, particularly in maize and soybean. Both cloning and T-RFLP indicated differences between AM communities in the different host species. Host species was more important than soil P in determining the AM community, except at the highest P concentration. Our results show that the impact of soil P on the diversity of AM fungi colonizing plants was broadly similar, despite the fact that different plants contained different communities. However, subtle differences in the response of the AM community in each host were evident.
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ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.12169