Tracking shallow chemical gradients by actin-driven wandering of the polarization site

Many cells are remarkably proficient at tracking very shallow chemical gradients, despite considerable noise from stochastic receptor-ligand interactions. Motile cells appear to undergo a biased random walk: spatial noise in receptor activity may determine the instantaneous direction, but because no...

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Vydáno v:	Current biology Ročník 23; číslo 1; s. 32
Hlavní autoři:	Dyer, Jayme M, Savage, Natasha S, Jin, Meng, Zyla, Trevin R, Elston, Timothy C, Lew, Daniel J
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England 07.01.2013
Témata:	Actins - metabolism Actins - physiology cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - analysis cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - metabolism cdc42 GTP-Binding Protein, Saccharomyces cerevisiae - physiology Cell Polarity Chemotaxis Pheromones - physiology Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - analysis Saccharomyces cerevisiae Proteins - metabolism Saccharomyces cerevisiae Proteins - physiology Time Factors
ISSN:	1879-0445, 1879-0445
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Popis
Shrnutí:	Many cells are remarkably proficient at tracking very shallow chemical gradients, despite considerable noise from stochastic receptor-ligand interactions. Motile cells appear to undergo a biased random walk: spatial noise in receptor activity may determine the instantaneous direction, but because noise is spatially unbiased, it is filtered out by time averaging, resulting in net movement upgradient. How nonmotile cells might filter out noise is unknown. Using yeast chemotropic mating as a model, we demonstrate that a polarized patch of polarity regulators "wanders" along the cortex during gradient tracking. Computational and experimental findings suggest that actin-directed membrane traffic contributes to wandering by diluting local polarity factors. The pheromone gradient appears to bias wandering via interactions between receptor-activated Gβγ and polarity regulators. Artificially blocking patch wandering impairs gradient tracking. We suggest that the polarity patch undergoes an intracellular biased random walk that enables noise filtering by time averaging, allowing nonmotile cells to track shallow gradients.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1879-0445 1879-0445
DOI:	10.1016/j.cub.2012.11.014