Light-triggered in vivo activation of adhesive peptides regulates cell adhesion, inflammation and vascularization of biomaterials

Materials engineered to elicit targeted cellular responses in regenerative medicine must display bioligands with precise spatial and temporal control. Although materials with temporally regulated presentation of bioadhesive ligands using external triggers, such as light and electric fields, have rec...

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Bibliographic Details
Published in:Nature materials Vol. 14; no. 3; pp. 352 - 360
Main Authors: Lee, Ted T., García, José R., Paez, Julieta I., Singh, Ankur, Phelps, Edward A., Weis, Simone, Shafiq, Zahid, Shekaran, Asha, del Campo, Aránzazu, García, Andrés J.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.03.2015
Nature Publishing Group
Subjects:
639/301/54
Activation
Adhesion
Adhesives
Animals
Biocompatibility
Biocompatible Materials - chemistry
Biomaterials
Biomedical materials
Cell adhesion
Cell adhesion & migration
Cell Adhesion - drug effects
Cell Adhesion - immunology
Cell Adhesion - radiation effects
Cell Adhesion Molecules - adverse effects
Cell Adhesion Molecules - radiation effects
Cell Line
Condensed Matter Physics
Drug Eruptions - immunology
Fibroblasts - drug effects
Fibroblasts - immunology
Fibroblasts - pathology
Humans
Hydrogels
In vivo testing
In vivo tests
Light
Male
Materials Science
Mice
Mice, Inbred BALB C
Nanotechnology
Neovascularization, Physiologic - drug effects
Neovascularization, Physiologic - immunology
NIH 3T3 Cells
Oligopeptides - adverse effects
Oligopeptides - radiation effects
Optical and Electronic Materials
Peptides
Studies
Surgical implants
ISSN:1476-1122, 1476-4660, 1476-4660
Online Access:Get full text
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Summary:Materials engineered to elicit targeted cellular responses in regenerative medicine must display bioligands with precise spatial and temporal control. Although materials with temporally regulated presentation of bioadhesive ligands using external triggers, such as light and electric fields, have recently been realized for cells in culture, the impact of in vivo temporal ligand presentation on cell–material responses is unknown. Here, we present a general strategy to temporally and spatially control the in vivo presentation of bioligands using cell-adhesive peptides with a protecting group that can be easily removed via transdermal light exposure to render the peptide fully active. We demonstrate that non-invasive, transdermal time-regulated activation of cell-adhesive RGD peptide on implanted biomaterials regulates in vivo cell adhesion, inflammation, fibrous encapsulation, and vascularization of the material. This work shows that triggered in vivo presentation of bioligands can be harnessed to direct tissue reparative responses associated with implanted biomaterials. Transdermal light-triggered activation of cell-adhesive peptides on the surface of implanted hydrogels alters cell–material interactions, such as cell adhesion and spatial patterning, and fibrous encapsulation and vascularization of the material.
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ISSN:1476-1122
1476-4660
1476-4660
DOI:10.1038/nmat4157