Light-triggered in vivo activation of adhesive peptides regulates cell adhesion, inflammation and vascularization of biomaterials

Materials engineered to elicit targeted cellular responses in regenerative medicine must display bioligands with precise spatial and temporal control. Although materials with temporally regulated presentation of bioadhesive ligands using external triggers, such as light and electric fields, have rec...

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Vydáno v:	Nature materials Ročník 14; číslo 3; s. 352 - 360
Hlavní autoři:	Lee, Ted T., García, José R., Paez, Julieta I., Singh, Ankur, Phelps, Edward A., Weis, Simone, Shafiq, Zahid, Shekaran, Asha, del Campo, Aránzazu, García, Andrés J.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.03.2015 Nature Publishing Group
Témata:	639/301/54 Activation Adhesion Adhesives Animals Biocompatibility Biocompatible Materials - chemistry Biomaterials Biomedical materials Cell adhesion Cell adhesion & migration Cell Adhesion - drug effects Cell Adhesion - immunology Cell Adhesion - radiation effects Cell Adhesion Molecules - adverse effects Cell Adhesion Molecules - radiation effects Cell Line Condensed Matter Physics Drug Eruptions - immunology Fibroblasts - drug effects Fibroblasts - immunology Fibroblasts - pathology Humans Hydrogels In vivo testing In vivo tests Light Male Materials Science Mice Mice, Inbred BALB C Nanotechnology Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - immunology NIH 3T3 Cells Oligopeptides - adverse effects Oligopeptides - radiation effects Optical and Electronic Materials Peptides Studies Surgical implants
ISSN:	1476-1122, 1476-4660, 1476-4660
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Shrnutí:	Materials engineered to elicit targeted cellular responses in regenerative medicine must display bioligands with precise spatial and temporal control. Although materials with temporally regulated presentation of bioadhesive ligands using external triggers, such as light and electric fields, have recently been realized for cells in culture, the impact of in vivo temporal ligand presentation on cell–material responses is unknown. Here, we present a general strategy to temporally and spatially control the in vivo presentation of bioligands using cell-adhesive peptides with a protecting group that can be easily removed via transdermal light exposure to render the peptide fully active. We demonstrate that non-invasive, transdermal time-regulated activation of cell-adhesive RGD peptide on implanted biomaterials regulates in vivo cell adhesion, inflammation, fibrous encapsulation, and vascularization of the material. This work shows that triggered in vivo presentation of bioligands can be harnessed to direct tissue reparative responses associated with implanted biomaterials. Transdermal light-triggered activation of cell-adhesive peptides on the surface of implanted hydrogels alters cell–material interactions, such as cell adhesion and spatial patterning, and fibrous encapsulation and vascularization of the material.
Bibliografie:	SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	1476-1122 1476-4660 1476-4660
DOI:	10.1038/nmat4157