Determining Lineage Pathways from Cellular Barcoding Experiments

Cellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in vivo cell fate that have been instrumental in several significant recent discoveries. Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the r...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cell reports (Cambridge) Jg. 6; H. 4; S. 617 - 624
Hauptverfasser: Perié, Leïla, Hodgkin, Philip D., Naik, Shalin H., Schumacher, Ton N., de Boer, Rob J., Duffy, Ken R.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 01.02.2014
Elsevier
Schlagworte:
Animals
Cell Lineage
Hematopoiesis
Hematopoietic Stem Cells - cytology
Life Sciences
Mice
Models, Biological
ISSN:2211-1247, 2211-1247
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Cellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in vivo cell fate that have been instrumental in several significant recent discoveries. Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the resulting data for evaluation of potential lineage pathways requires a new quantitative framework complete with appropriate statistical tests. Here, we develop such a framework, illustrating its utility by analyzing data from barcoded multipotent cells of the blood system. This application demonstrates that the data require additional paths beyond those found in the classical model, which leads us to propose that hematopoietic differentiation follows a loss of potential mechanism and to suggest further experiments to test this deduction. Our quantitative framework can evaluate the compatibility of lineage trees with barcoded data from any proliferating and differentiating cell system. [Display omitted] •A general framework for lineage pathway inference from cellular barcoding is described•Application to hematopoiesis reveals that the classical model is incomplete•A consistent lineage pathway between mice is identified•Inclusion of all potential differentiation links is required to explain the data Cellular barcoding and other single-cell lineage tracing strategies form experimental methodologies for analysis of in vivo cell fate. Perié and colleagues have developed a quantitative framework to identify potential differentiation pathways that are statistically consistent with cellular barcoding data and other lineage tracing strategies. Using this framework, they demonstrate that the hematopoietic pathway requires additional branches beyond those found in the most commonly held model of hematopoiesis, leading them to propose a revised model.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2014.01.016