Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations

Next-generation sequencing approaches in microbiome research have allowed surveys of microbial communities, their genomes, and their functions with higher sensitivity than ever before. However, this sensitivity is a double-edged sword because these tools also efficiently detect contaminant DNA and c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Trends in microbiology (Regular ed.) Jg. 27; H. 2; S. 105 - 117
Hauptverfasser:	Eisenhofer, Raphael, Minich, Jeremiah J., Marotz, Clarisse, Cooper, Alan, Knight, Rob, Weyrich, Laura S.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	England Elsevier Ltd 01.02.2019 Elsevier Science Ltd
Schlagworte:	Biomass Contaminants Contamination cross contamination Deoxyribonucleic acid DNA DNA Contamination DNA, Bacterial - analysis DNA, Bacterial - genetics genome Genomes High-Throughput Nucleotide Sequencing Humans laboratory contamination low biomass Metagenomics methodology Microbial activity microbial biomass microbial communities Microbial contamination Microbiological Techniques - methods Microbiological Techniques - standards microbiome Microbiomes microbiota Microbiota - genetics Microorganisms Next-generation sequencing Reproducibility of Results Sensitivity Specimen Handling surveys microbiota laboratory contamination DNA contamination microbiome methodology low biomass
ISSN:	0966-842X, 1878-4380, 1878-4380
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!