Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors

The development of new CRISPR-Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currently available for modifying genomes in experimental s...

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Bibliographic Details
Published in:Nature biotechnology Vol. 38; no. 7; pp. 824 - 844
Main Authors: Anzalone, Andrew V, Koblan, Luke W, Liu, David R
Format: Journal Article
Language:English
Published: United States Nature Publishing Group 01.07.2020
Subjects:
CRISPR
CRISPR-Cas Systems - genetics
DNA Breaks, Double-Stranded
Editing
Endonucleases - genetics
Gene Editing - methods
Genome - genetics
Genome editing
Genomes
Humans
Nuclease
Recombinases - genetics
Transposases - genetics
ISSN:1087-0156, 1546-1696, 1546-1696
Online Access:Get full text
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Summary:The development of new CRISPR-Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currently available for modifying genomes in experimental systems. Some of these agents have also moved rapidly into the clinic. Each tool comes with its own capabilities and limitations, and major efforts have broadened their editing capabilities, expanded their targeting scope and improved editing specificity. We analyze key considerations when choosing genome editing agents and identify opportunities for future improvements and applications in basic research and therapeutics.
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ISSN:1087-0156
1546-1696
1546-1696
DOI:10.1038/s41587-020-0561-9