Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors

The development of new CRISPR-Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currently available for modifying genomes in experimental s...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Nature biotechnology Ročník 38; číslo 7; s. 824 - 844
Hlavní autoři: Anzalone, Andrew V, Koblan, Luke W, Liu, David R
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Nature Publishing Group 01.07.2020
Témata:
CRISPR
CRISPR-Cas Systems - genetics
DNA Breaks, Double-Stranded
Editing
Endonucleases - genetics
Gene Editing - methods
Genome - genetics
Genome editing
Genomes
Humans
Nuclease
Recombinases - genetics
Transposases - genetics
ISSN:1087-0156, 1546-1696, 1546-1696
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:The development of new CRISPR-Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currently available for modifying genomes in experimental systems. Some of these agents have also moved rapidly into the clinic. Each tool comes with its own capabilities and limitations, and major efforts have broadened their editing capabilities, expanded their targeting scope and improved editing specificity. We analyze key considerations when choosing genome editing agents and identify opportunities for future improvements and applications in basic research and therapeutics.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ObjectType-Review-3
content type line 23
ISSN:1087-0156
1546-1696
1546-1696
DOI:10.1038/s41587-020-0561-9