Accuracy of QuantiFERON SARS-CoV-2 research use only assay and characterization of the CD4+ and CD8+ T cell-SARS-CoV-2 response: comparison with a homemade interferon-γ release assay

•The QuantiFERON SARS-CoV-2 research use only assay response is mediated by CD4+ and CD8+ T cells.•Immune response to the antigen (Ag)2 tube is more accurate than that to the Ag1 tube.•Responses to QuantiFERON SARS-CoV-2 tubes are lower compared with a homemade test.•The QuantiFERON SARS-CoV-2 diffe...

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Vydané v:International journal of infectious diseases Ročník 122; s. 841 - 849
Hlavní autori: Aiello, Alessandra, Coppola, Andrea, Vanini, Valentina, Petrone, Linda, Cuzzi, Gilda, Salmi, Andrea, Altera, Anna Maria Gerarda, Tortorella, Carla, Gualano, Gina, Gasperini, Claudio, Scolieri, Palma, Beccacece, Alessia, Vita, Serena, Bruzzese, Vincenzo, Lorenzetti, Roberto, Palmieri, Fabrizio, Nicastri, Emanuele, Goletti, Delia
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Canada Elsevier Ltd 01.09.2022
The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases
Elsevier
Predmet:
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
COVID-19
COVID-19 - diagnosis
Humans
IFN-γ release assay (IGRA), T cell response
Interferon-gamma Release Tests
Mycobacterium tuberculosis
QuantiFERON SARS-CoV-2 tubes
SARS-CoV-2
Spike peptides
Tuberculosis
Whole-blood
COVID-19
QuantiFERON SARS-CoV-2 tubes
Whole-blood
Spike peptides
IFN-γ release assay (IGRA), T cell response
ISSN:1201-9712, 1878-3511, 1878-3511
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Shrnutí:•The QuantiFERON SARS-CoV-2 research use only assay response is mediated by CD4+ and CD8+ T cells.•Immune response to the antigen (Ag)2 tube is more accurate than that to the Ag1 tube.•Responses to QuantiFERON SARS-CoV-2 tubes are lower compared with a homemade test.•The QuantiFERON SARS-CoV-2 difference in accuracy is likely because of the peptide composition. Objectives: In this study, we aimed to characterize the SARS-CoV-2-specific T cell response detected by the QuantiFERON SARS-CoV-2 research use only assay in terms of accuracy and T cell subsets involved compared with a homemade interferon (IFN)-γ release assay (IGRA). Methods: We evaluated T cell response by the standardized QuantiFERON SARS-CoV-2 tubes (antigen [Ag]1 and Ag2) and a homemade IGRA quantifying IFN-γ response to SARS-CoV-2 spike peptides (homemade-IGRA-SPIKE test). We evaluated the T cell subsets mediating the specific response using flow cytometry. Results: We prospectively enrolled 66 individuals: COVID-19 or post-COVID-19 subjects and NO-COVID-19-vaccinated subjects, including healthy donors and immunocompromised subjects. The standardized kit detected 62.1% (41/66) of T cell responders. Ag2 tube showed a higher IFN-γ quantitative and qualitative response. Ag1 tube response was mainly mediated by CD4+ T cells; Ag2 tube response was mediated by CD4+ and CD8+ T cells. The homemade-IGRA-SPIKE test detected a higher number of responders (52/66, 78.8%) than the QuantiFERON SARS-CoV-2 assay (P = 0.056). The response was found in both T cell subsets, although a higher magnitude and response rate was observed in the CD4+ T cell subset. Conclusion: The QuantiFERON SARS-CoV-2 response is mediated by CD4+ and CD8+ T cells. A lower number of responders is found compared with the homemade-IGRA-SPIKE test, likely because of the different peptide composition.
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These authors have contributed equally to this work and share first authorship.
ISSN:1201-9712
1878-3511
1878-3511
DOI:10.1016/j.ijid.2022.07.049