PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Vydané v:	Biology open Ročník 5; číslo 7; s. 1001 - 1009
Hlavní autori:	Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	England The Company of Biologists Ltd 15.07.2016 The Company of Biologists
Predmet:	Actin Actin cytoskeleton Cellular structure Clathrin Cytoskeleton dSTORM Fixation Image resolution Lamellipodia Localization Methods & Techniques Organelles Protein localization Proteins Pseudopodia Regulatory proteins Reproducibility Sample preparation Super-resolution microscopy (SRM) Protein localization dSTORM Fixation Actin cytoskeleton Super-resolution microscopy (SRM)
ISSN:	2046-6390, 2046-6390
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