Bioactive Peptide Brush Polymers via Photoinduced Reversible‐Deactivation Radical Polymerization

Harnessing metal‐free photoinduced reversible‐deactivation radical polymerization (photo‐RDRP) in organic and aqueous phases, we report a synthetic approach to enzyme‐responsive and pro‐apoptotic peptide brush polymers. Thermolysin‐responsive peptide‐based polymeric amphiphiles assembled into spheri...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Angewandte Chemie International Edition Ročník 58; číslo 48; s. 17359 - 17364
Hlavní autoři:	Sun, Hao, Choi, Wonmin, Zang, Nanzhi, Battistella, Claudia, Thompson, Matthew P., Cao, Wei, Zhou, Xuhao, Forman, Christopher, Gianneschi, Nathan C.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	WEINHEIM Wiley 25.11.2019 Wiley Subscription Services, Inc
Vydání:	International ed. in English
Témata:	Acrylates - chemistry Amino Acids - chemistry Apoptosis Biocompatibility Biological activity biomaterials Brushes Cell Membrane Permeability - drug effects Cell Survival - drug effects Chemistry Chemistry, Multidisciplinary Cytotoxicity Deactivation Ductile-brittle transition Enzymes Fracture mechanics Free Radicals - chemistry HeLa Cells Humans Micelles Molecular Conformation Morphology nanomaterials Nanoparticles Nanoparticles - chemistry peptide brush polymer Peptides Peptides - chemistry Photochemical Processes photochemistry Physical Sciences Polymerization Polymers Polymers - chemistry Polymethyl Methacrylate - chemistry Science & Technology Solvents - chemistry Structure-Activity Relationship Thermolysin Thermolysin - chemistry Toxicity OXYGEN polymerization MECHANISM ROBUST photochemistry nanomaterials peptide brush polymer biomaterials
ISSN:	1433-7851, 1521-3773, 1521-3773
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Harnessing metal‐free photoinduced reversible‐deactivation radical polymerization (photo‐RDRP) in organic and aqueous phases, we report a synthetic approach to enzyme‐responsive and pro‐apoptotic peptide brush polymers. Thermolysin‐responsive peptide‐based polymeric amphiphiles assembled into spherical micellar nanoparticles that undergo a morphology transition to worm‐like micelles upon enzyme‐triggered cleavage of coronal peptide sidechains. Moreover, pro‐apoptotic polypeptide brushes show enhanced cell uptake over individual peptide chains of the same sequence, resulting in a significant increase in cytotoxicity to cancer cells. Critically, increased grafting density of pro‐apoptotic peptides on brush polymers correlates with increased uptake efficiency and concurrently, cytotoxicity. The mild synthetic conditions afforded by photo‐RDRP, make it possible to access well‐defined peptide‐based polymer bioconjugate structures with tunable bioactivity. Empowered by light: Photoinduced reversible deactivation radical polymerization (photo‐RDRP) enabled access to a new class of bioactive peptide brush polymers. Those peptide‐based brush polymers exhibited well‐defined structures and tunable bioactivities, highlighting their immense potential in biomedical applications.
Bibliografie:	These authors contributed equally to this work. NIH RePORTER ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 H.S., W. Choi, and N.C.G. conceived the project and designed the experiments. H.S. and W. Choi. synthesized and purified the materials. H.S. and X.Z. performed the TEM analysis. C.F. performed the AFM characterization. W. Choi. and N.Z. conducted the cell study. N.Z. and C.B. conducted confocal laser scanning microscopy. N.Z., W. Cao., and H.S. performed the flow cytometry. H.S., W. Choi., M.P.T., and N.C. co-wrote the manuscript. All the authors discussed the results on the manuscript. Those authors contributed equally to the work. Contributions
ISSN:	1433-7851 1521-3773 1521-3773
DOI:	10.1002/anie.201908634