Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater

[Display omitted] •Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR demonstrated superior analytical sensitivity.•RT-ddPCR was resistant to inhibition in a wastewater matrix and was reproducible across laborat...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 297; p. 114230
Main Authors:	Ciesielski, Mark, Blackwood, Denene, Clerkin, Thomas, Gonzalez, Raul, Thompson, Hannah, Larson, Allison, Noble, Rachel
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.11.2021
Subjects:	COVID-19 detection limit Humans Molecular methods monitoring North Carolina nucleic acids nucleocapsid proteins pandemic pathogens PCR Real-Time Polymerase Chain Reaction Reproducibility of Results RNA, Viral - genetics sanitation SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Virginia Wastewater North Carolina Virginia SARS-CoV-2 Wastewater Molecular methods PCR
ISSN:	0166-0934, 1879-0984, 1879-0984
Online Access:	Get full text
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