SARS-CoV-2: Comparative analysis of different RNA extraction methods

[Display omitted] •Alternative suitable methods to extract SARS-CoV-2 RNA should be taken into account to cope with the ongoing pandemic.•Extraction efficiencies of different viral RNA kit brands can be optimized for detection of SARS-CoV-2 genome.•The accuracy and performance of the Allplex 2019-nC...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 287; p. 114008
Main Authors:	Ambrosi, Cecilia, Prezioso, Carla, Checconi, Paola, Scribano, Daniela, Sarshar, Meysam, Capannari, Maurizio, Tomino, Carlo, Fini, Massimo, Garaci, Enrico, Palamara, Anna Teresa, De Chiara, Giovanna, Limongi, Dolores
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.01.2021 The Author(s). Published by Elsevier B.V
Subjects:	COVID-19 - diagnosis COVID-19 infection COVID-19 Nucleic Acid Testing - methods Diagnostic Tests, Routine etiological agents Genes, Viral - genetics Humans Limit of Detection Oro- nasopharyngeal swabs Pharynx - virology quantitative polymerase chain reaction Reproducibility of Results reverse transcription RNA RNA extraction RNA, Viral - analysis RNA, Viral - genetics RNA, Viral - isolation & purification rRT-PCR SARS-CoV-2 SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification separation Severe acute respiratory syndrome coronavirus 2 RNA extraction SARS-CoV-2 Oro- nasopharyngeal swabs rRT-PCR
ISSN:	0166-0934, 1879-0984, 1879-0984
Online Access:	Get full text
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