High-Throughput Sequencing Reveals Three Rhabdoviruses Persisting in the IRE/CTVM19 Cell Line

Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to...

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Vydáno v:	Viruses Ročník 16; číslo 4; s. 576
Hlavní autoři:	Litov, Alexander G., Shchetinin, Alexey M., Kholodilov, Ivan S., Belova, Oxana A., Gadzhikurbanov, Magomed N., Ivannikova, Anna Y., Kovpak, Anastasia A., Gushchin, Vladimir A., Karganova, Galina G.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland MDPI AG 09.04.2024 MDPI
Témata:	Analysis Animals Arachnids Bacteria Biology Cell culture Cell Line Cell lines Embryos endogenous virus Genetic aspects genome Genome, Viral Genomes High-Throughput Nucleotide Sequencing Host-virus relationships Identification and classification IRE/CTVM19 IRE/CTVM19-associated rhabdovirus Ixodes ricinus mammals Microscopy Next-generation sequencing Norway Pathogens persistence Phylogenetics Phylogeny Plant viruses Rhabdoviridae Rhabdoviridae - classification Rhabdoviridae - genetics Rhabdoviridae - isolation & purification Rhabdoviridae Infections - veterinary Rhabdoviridae Infections - virology Rhabdoviruses RNA RNA polymerase RNA, Viral - genetics Software tick cell line Ticks Virome - genetics Virus Replication Viruses Russia United Kingdom > UK Lithuania United States > US Norway Germany Rhabdoviridae IRE/CTVM19-associated rhabdovirus Norway mononegavirus 1 Chimay rhabdovirus endogenous virus persistence tick cell line IRE/CTVM19
ISSN:	1999-4915, 1999-4915
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Shrnutí:	Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to rhabdoviruses and iflaviruses, were detected in the IRE/CTVM19 cell line, suggesting the presence of multiple persisting viruses. Subsequently, the full genome of an IRE/CTVM19-associated rhabdovirus was recovered from a cell culture during the isolation of the Alongshan virus. In the current work, we used high-throughput sequencing to describe a virome of the IRE/CTVM19 cell line. In addition to the previously detected IRE/CTVM19-associated rhabdovirus, two rhabdoviruses were detected: Chimay rhabdovirus and Norway mononegavirus 1. In the follow-up experiments, we were able to detect both positive and negative RNA strands of the IRE/CTVM19-associated rhabdovirus and Norway mononegavirus 1 in the IRE/CTVM19 cells, suggesting their active replication in the cell line. Passaging attempts in cell lines of mammalian origin failed for all three discovered rhabdoviruses.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v16040576