High-Throughput Sequencing Reveals Three Rhabdoviruses Persisting in the IRE/CTVM19 Cell Line

Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to...

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Bibliographic Details
Published in:Viruses Vol. 16; no. 4; p. 576
Main Authors: Litov, Alexander G., Shchetinin, Alexey M., Kholodilov, Ivan S., Belova, Oxana A., Gadzhikurbanov, Magomed N., Ivannikova, Anna Y., Kovpak, Anastasia A., Gushchin, Vladimir A., Karganova, Galina G.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 09.04.2024
MDPI
Subjects:
Analysis
Animals
Arachnids
Bacteria
Biology
Cell culture
Cell Line
Cell lines
Embryos
endogenous virus
Genetic aspects
genome
Genome, Viral
Genomes
High-Throughput Nucleotide Sequencing
Host-virus relationships
Identification and classification
IRE/CTVM19
IRE/CTVM19-associated rhabdovirus
Ixodes ricinus
mammals
Microscopy
Next-generation sequencing
Norway
Pathogens
persistence
Phylogenetics
Phylogeny
Plant viruses
Rhabdoviridae
Rhabdoviridae - classification
Rhabdoviridae - genetics
Rhabdoviridae - isolation & purification
Rhabdoviridae Infections - veterinary
Rhabdoviridae Infections - virology
Rhabdoviruses
RNA
RNA polymerase
RNA, Viral - genetics
Software
tick cell line
Ticks
Virome - genetics
Virus Replication
Viruses
Russia
United Kingdom > UK
Lithuania
United States > US
Norway
Germany
Rhabdoviridae
IRE/CTVM19-associated rhabdovirus
Norway mononegavirus 1
Chimay rhabdovirus
endogenous virus
persistence
tick cell line
IRE/CTVM19
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Description
Summary:Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to rhabdoviruses and iflaviruses, were detected in the IRE/CTVM19 cell line, suggesting the presence of multiple persisting viruses. Subsequently, the full genome of an IRE/CTVM19-associated rhabdovirus was recovered from a cell culture during the isolation of the Alongshan virus. In the current work, we used high-throughput sequencing to describe a virome of the IRE/CTVM19 cell line. In addition to the previously detected IRE/CTVM19-associated rhabdovirus, two rhabdoviruses were detected: Chimay rhabdovirus and Norway mononegavirus 1. In the follow-up experiments, we were able to detect both positive and negative RNA strands of the IRE/CTVM19-associated rhabdovirus and Norway mononegavirus 1 in the IRE/CTVM19 cells, suggesting their active replication in the cell line. Passaging attempts in cell lines of mammalian origin failed for all three discovered rhabdoviruses.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v16040576