B lymphocyte inhibition of anti-tumor response depends on expansion of Treg but is independent of B-cell IL-10 secretion

The mechanisms by which B lymphocytes inhibit anti-tumor immunity remain poorly understood. Murine EMT-6 mammary tumors grow readily in immune competent mice (BALB/c), but poorly in B-cell-deficient μ −/− BALB/c mice (BCDM). T regulatory cell (Treg) expansion and function were impaired in BCDM compa...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Cancer Immunology, Immunotherapy Ročník 62; číslo 1; s. 87 - 99
Hlavní autoři: Zhang, Yu, Eliav, Yair, Shin, Seung-uon, Schreiber, Taylor H., Podack, Eckhard R., Tadmor, Tamar, Rosenblatt, Joseph D.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Berlin/Heidelberg Springer-Verlag 01.01.2013
Springer Nature B.V
Témata:
Adoptive Transfer
Animals
Antibodies
Antigens
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Cancer
Cancer Research
CD8 antigen
Cell growth
Cell Proliferation
Cytokines
Cytotoxicity
Flow Cytometry
Immunology
Immunoregulation
Immunosuppression
Immunotherapy
Interleukin 10
Interleukin-10 - immunology
Interleukin-10 - metabolism
Laboratories
Lymphocyte Activation - immunology
Lymphocytes
Lymphocytes B
Lymphocytes T
Lymphocytes, Tumor-Infiltrating - immunology
Mammary gland
Mammary Neoplasms, Experimental - immunology
Medicine
Medicine & Public Health
Metastases
Mice
Mice, Inbred BALB C
Mice, Knockout
Microenvironments
Oncology
Original
Original Article
Receptor Cross-Talk - immunology
T-Lymphocytes, Regulatory - cytology
T-Lymphocytes, Regulatory - immunology
Tumor Microenvironment - immunology
Tumors
United States > US
B lymphocyte
T regulatory cells (Treg)
B-cell-deficient mice (BCDM)
IL-10
Immune competent mice (BALB/c)
ISSN:0340-7004, 1432-0851, 1432-0851
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:The mechanisms by which B lymphocytes inhibit anti-tumor immunity remain poorly understood. Murine EMT-6 mammary tumors grow readily in immune competent mice (BALB/c), but poorly in B-cell-deficient μ −/− BALB/c mice (BCDM). T regulatory cell (Treg) expansion and function were impaired in BCDM compared with BALB/c. In this study, we compared tumor growth, Treg cell proliferation, tumor lymphocyte infiltration and cytolytic T cell activity in BALB/c, BCDM and BCDM partially reconstituted with B cells by adoptive transfer (BCDM+B). Partial reconstitution of BCDM with adoptively transferred B cells restored EMT-6 tumor growth, which was independent of IL-10 secretion by B cells. Instead, high frequencies of intratumoral B cells were associated with increased recruitment and proliferation of Treg cells within the tumor microenvironment. The B-cell-dependent accumulation of Treg within the tumor microenvironment was associated with reduced tumor infiltration by CD49+ NK and CD8+ T cells and reduced cytotoxic T cell activity against EMT-6 targets. Our studies indicate that tumor-dependent immunosuppression of T-cell-mediated anti-tumor immunity is coordinated within the tumor microenvironment by B-cell-dependent cross talk with Treg cells, which does not require production of IL-10 by B cells.
Bibliografie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ObjectType-Article-1
ObjectType-Feature-2
content type line 23
ISSN:0340-7004
1432-0851
1432-0851
DOI:10.1007/s00262-012-1313-6