Methanol induces cytosolic calcium variations, membrane depolarization and ethylene production in arabidopsis and tobacco

Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, mo...

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Vydané v:Annals of botany Ročník 122; číslo 5; s. 849
Hlavní autori: Tran, Daniel, Dauphin, Aurélien, Meimoun, Patrice, Kadono, Takashi, Nguyen, Hieu T H, Arbelet-Bonnin, Delphine, Zhao, Tingting, Errakhi, Rafik, Lehner, Arnaud, Kawano, Tomonori, Bouteau, François
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 03.11.2018
Predmet:
Aequorin - metabolism
Apoproteins - metabolism
Arabidopsis - drug effects
Arabidopsis - physiology
Calcium - metabolism
Cell Membrane - physiology
Cells, Cultured
Cytosol - metabolism
Ethylenes - metabolism
Methanol - administration & dosage
Nicotiana - drug effects
Nicotiana - physiology
Plant Growth Regulators - metabolism
Plant Proteins - metabolism
Reactive Oxygen Species - metabolism
Recombinant Proteins - metabolism
Seedlings - drug effects
Seedlings - physiology
ISSN:1095-8290, 1095-8290
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Shrnutí:Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. Methanol induces cytosolic Ca2+ variations that involve Ca2+ influx through the plasma membrane and Ca2+ release from internal stores. Our data further suggest that these Ca2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. Methanol is not only a by-product of PME activities, and our data suggest that [Ca2+]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1095-8290
1095-8290
DOI:10.1093/aob/mcy038