Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19

•Isothermal, rapid amplification and lateral flow qualitative detection of SARS-CoV-2.•Two-step, point-of-care diagnostic development for COVID-19.•Sensitive/specific assay that doesn’t require sophisticated equipment or training.•Able to detect 0.25–2.5 copies/μL of SARS-CoV-2 N gene containing pla...

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Vydané v:	Journal of virological methods Ročník 296; s. 114227
Hlavní autori:	Shelite, Thomas R., Uscanga-Palomeque, Ashanti C., Castellanos-Gonzalez, Alejandro, Melby, Peter C., Travi, Bruno L.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Netherlands Elsevier B.V 01.10.2021 Elsevier/North-Holland Biomedical Press
Predmet:	Coronavirus COVID-19 - diagnosis COVID-19 - virology COVID-19 infection COVID-19 Nucleic Acid Testing - methods detection limit Diagnostic Tests, Routine Diagnostics DNA Primers genes Humans Lateral flow mortality Nucleic Acid Amplification Techniques - methods nucleocapsid people plasmids Point-of-care point-of-care systems Point-of-Care Testing rapid methods Real-Time Polymerase Chain Reaction - methods recombinases Recombinases - chemistry RNA, Viral - genetics RPA SARS-CoV-2 SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Sensitivity and Specificity Severe acute respiratory syndrome coronavirus 2 viruses SARS-CoV-2 RPA Coronavirus Lateral flow Diagnostics Point-of-care
ISSN:	0166-0934, 1879-0984, 1879-0984
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