Identification of Arabidopsis thaliana NRT1/PTR FAMILY (NPF) proteins capable of transporting plant hormones

NRT1/PTR FAMILY (NPF) proteins were originally identified as nitrate or di/tri-peptide transporters. Recent studies revealed that th is transporter family also transports the plant hormones auxin (indole-3-acetic acid), abscisic acid (ABA), and gibberellin (GA), as well as secondary metabolites ( gl...

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Vydané v:Journal of plant research Ročník 128; číslo 4; s. 679 - 686
Hlavní autori: Chiba, Yasutaka, Shimizu, Takafumi, Miyakawa, Shinya, Kanno, Yuri, Koshiba, Tomokazu, Kamiya, Yuji, Seo, Mitsunori
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Tokyo Springer Japan 01.07.2015
Springer Nature B.V
Predmet:
abscisic acid
Acetic acid
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biomedical and Life Sciences
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cloning, Molecular
Flowers & plants
Gene Expression Regulation, Plant - physiology
gibberellins
glucosinolates
Hormones
indole acetic acid
Life Sciences
Liquid chromatography
Mass spectrometry
Metabolites
Multigene Family
nitrates
Peptides
Plant Biochemistry
Plant Ecology
Plant Growth Regulators - metabolism
Plant Physiology
Plant Sciences
protein synthesis
Proteins
Regular Paper
Secondary metabolites
tandem mass spectrometry
transporters
Two-Hybrid System Techniques
Yeasts
Abscisic acid (ABA)
Arabidopsis
Jasmonoyl-isoleucine (JA-Ile)
)
NRT1/PTR FAMILY (NPF)
Transport
Gibberellin (GA)
ISSN:0918-9440, 1618-0860
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Shrnutí:NRT1/PTR FAMILY (NPF) proteins were originally identified as nitrate or di/tri-peptide transporters. Recent studies revealed that th is transporter family also transports the plant hormones auxin (indole-3-acetic acid), abscisic acid (ABA), and gibberellin (GA), as well as secondary metabolites ( glucosinolates). We developed modified yeast two-hybrid systems with receptor complexes for GA and jasmonoyl-isoleucine (JA-Ile), to detect GA and JA-Ile transport activities of proteins expressed in the yeast cells. Using these GA and JA-Ile systems as well as the ABA system that we had introduced previously, we determined the capacities of Arabidopsis NPFs to transport these hormones. Several NPFs induced the formation of receptor complexes under relatively low hormone concentrations. Hormone transport activities were confirmed for some NPFs by direct analysis of hormone uptake of yeast cells by liquid chromatography–tandem mass spectrometry. Our results suggest that at least some NPFs could function as hormone transporters.
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ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-015-0710-2