Propagation, Inactivation, and Safety Testing of SARS-CoV-2

In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandem...

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Vydáno v:	Viruses Ročník 12; číslo 6; s. 622
Hlavní autoři:	Jureka, Alexander, Silvas, Jesus, Basler, Christopher
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland MDPI AG 06.06.2020 MDPI
Témata:	agarose Animals Betacoronavirus - drug effects Betacoronavirus - growth & development Betacoronavirus - pathogenicity Betacoronavirus - physiology biosafety Cell lines Cellulose China Chlorocebus aethiops Coronaviridae coronavirus Coronavirus Infections - virology Coronaviruses COVID-19 COVID-19 infection Culture Media - chemistry DNA Formaldehyde formalin Gene expression Genomes Guanidines - pharmacology Heat HEK293 Cells Humans inactivation Infections Infectivity lactones pandemic Pandemics pathogenicity Phenols - pharmacology Plaque assay Pneumonia, Viral - virology Propagation Propiolactone - pharmacology Ribonucleic acid RNA safety testing SARS-CoV-2 Sepharose Severe acute respiratory syndrome coronavirus 2 Vero Cells Viral Plaque Assay - methods virology virus Virus Inactivation Viruses United States > US China coronavirus SARS-CoV-2 virology virus plaque assay inactivation
ISSN:	1999-4915, 1999-4915
On-line přístup:	Získat plný text
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Shrnutí:	In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandemic has instigated widespread research related to SARS-CoV-2 and the disease that it causes, COVID-19. Research into this new virus will be facilitated by the availability of clearly described and effective procedures that enable the propagation and quantification of infectious virus. As work with the virus is recommended to be performed at biosafety level 3, validated methods to effectively inactivate the virus to enable the safe study of RNA, DNA, and protein from infected cells are also needed. Here, we report methods used to grow SARS-CoV-2 in multiple cell lines and to measure virus infectivity by plaque assay using either agarose or microcrystalline cellulose as an overlay as well as a SARS-CoV-2 specific focus forming assay. We also demonstrate effective inactivation by TRIzol, 10% neutral buffered formalin, beta propiolactone, and heat.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Those authors contributed equally to this manuscript.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v12060622