Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection

One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the...

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Veröffentlicht in:	Nature protocols Jg. 5; H. 12; S. 1983 - 1992
Hauptverfasser:	Skidmore, Mark A, Guimond, Scott E, Dumax-Vorzet, Audrey F, Yates, Edwin A, Turnbull, Jeremy E
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Nature Publishing Group UK 01.12.2010 Nature Publishing Group
Schlagworte:	631/1647/2230/1378 631/1647/666/2260 631/45/72/1205 Analytical Chemistry Anticoagulants Biological Techniques Biomedical and Life Sciences Boron Compounds Carbohydrates Chromatography, High Pressure Liquid - methods Composition Computational Biology/Bioinformatics Disaccharides - isolation & purification Enzymes Fluorescence Fluorescent Dyes Heparan sulfate Heparin Heparin - analysis Heparitin Sulfate - analysis High performance liquid chromatography Life Sciences Liquid chromatography Methods Microarrays Organic Chemistry Properties protocol Saccharides Spectrophotometry, Ultraviolet - methods Sugars Sulfates United Kingdom United Kingdom > UK
ISSN:	1754-2189, 1750-2799, 1750-2799
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Abstract	One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the initial characterization of spatially and temporally regulated structures. Two protocols for conducting disaccharide analysis are presented here, both exploiting exhaustive digestion of the polysaccharide, yielding constituent disaccharides, by bacterial heparin lyases. The first method, suitable for microgram quantities of material, relies on the separation of the disaccharides by high-performance liquid chromatography (HPLC) coupled to ultraviolet absorbance detection and can be performed in 2 d. The second exploits reducing end–labeling with the fluorophore BODIPY hydrazide, separation by HPLC, and subsequent fluorescence detection and quantitation. The latter is a high-sensitivity method that requires nanograms of starting material and has a detection limit in the low fmol range, and is thus the most sensitive method for disaccharide compositional analysis of HS yet reported. Fluorescence detection can be routinely carried out in 3 d.
AbstractList	One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the initial characterization of spatially and temporally regulated structures. Two protocols for conducting disaccharide analysis are presented here, both exploiting exhaustive digestion of the polysaccharide, yielding constituent disaccharides, by bacterial heparin lyases. The first method, suitable for microgram quantities of material, relies on the separation of the disaccharides by high-performance liquid chromatography (HPLC) coupled to ultraviolet absorbance detection and can be performed in 2 d. The second exploits reducing end-labeling with the fluorophore BODIPY hydrazide, separation by HPLC, and subsequent fluorescence detection and quantitation. The latter is a high-sensitivity method that requires nanograms of starting material and has a detection limit in the low fmol range, and is thus the most sensitive method for disaccharide compositional analysis of HS yet reported. Fluorescence detection can be routinely carried out in 3 d. One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the initial characterization of spatially and temporally regulated structures. Two protocols for conducting disaccharide analysis are presented here, both exploiting exhaustive digestion of the polysaccharide, yielding constituent disaccharides, by bacterial heparin lyases. The first method, suitable for microgram quantities of material, relies on the separation of the disaccharides by high-performance liquid chromatography (HPLC) coupled to ultraviolet absorbance detection and can be performed in 2 d. The second exploits reducing end-labeling with the fluorophore BODIPY hydrazide, separation by HPLC, and subsequent fluorescence detection and quantitation. The latter is a high-sensitivity method that requires nanograms of starting material and has a detection limit in the low fmol range, and is thus the most sensitive method for disaccharide compositional analysis of HS yet reported. Fluorescence detection can be routinely carried out in 3 d.One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the initial characterization of spatially and temporally regulated structures. Two protocols for conducting disaccharide analysis are presented here, both exploiting exhaustive digestion of the polysaccharide, yielding constituent disaccharides, by bacterial heparin lyases. The first method, suitable for microgram quantities of material, relies on the separation of the disaccharides by high-performance liquid chromatography (HPLC) coupled to ultraviolet absorbance detection and can be performed in 2 d. The second exploits reducing end-labeling with the fluorophore BODIPY hydrazide, separation by HPLC, and subsequent fluorescence detection and quantitation. The latter is a high-sensitivity method that requires nanograms of starting material and has a detection limit in the low fmol range, and is thus the most sensitive method for disaccharide compositional analysis of HS yet reported. Fluorescence detection can be routinely carried out in 3 d.
Audience	Academic
Author	Yates, Edwin A Dumax-Vorzet, Audrey F Turnbull, Jeremy E Guimond, Scott E Skidmore, Mark A
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Cites_doi	10.1182/blood-2002-07-2016 10.1042/bj2730553 10.1016/S0962-8924(00)01897-3 10.1038/nmeth807 10.1021/bi00462a026 10.1038/nrd724 10.1006/abio.1996.0352 10.1016/j.molcel.2004.10.008 10.1016/S0960-9822(00)80060-3 10.1016/S0021-9673(98)00854-1 10.1093/glycob/cwh051 10.1006/abio.1999.4027 10.1083/jcb.200303059 10.1002/1615-9861(200102)1:2<304::AID-PROT304>3.0.CO;2-B 10.1074/jbc.M109.032755 10.1038/nature05817 10.1073/pnas.96.6.2698 10.1038/nprot.2010.17 10.1038/nprot.2010.48 10.1039/b100916h 10.1021/jm701337t 10.1093/glycob/cwn028 10.1016/j.chroma.2006.09.064 10.1038/nchembio0207-74 10.1093/glycob/cwf072 10.1016/j.chroma.2008.06.007 10.1016/B978-008044859-6/50007-1
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References	Drummond, Yates, Turnbull (CR22) 2001; 1 Toyoda, Yamamoto, Ogino, Toida, Imanari (CR26) 1999; 830 Ambrosius, Kleesiek, Gotting (CR28) 2008; 1201 Turnbull, Powell, Guimond (CR4) 2001; 11 Bishop, Schuksz, Esko (CR3) 2007; 446 Guimond, Turnbull (CR14) 1999; 9 Turnbull, Hopwood, Gallagher (CR20) 1999; 96 Huang (CR24) 1996; 240 Powell, Yates, Fernig, Turnbull (CR13) 2004; 14 Turnbull, Gallagher (CR7) 1991; 273 Turnbull, Field (CR2) 2007; 3 Deakin, Lyon (CR21) 2008; 18 Skidmore, Atrih, Yates (CR29) 2009; 534 Skidmore (CR9) 2008; 51 Powell, Ahmed, Yates, Turnbull (CR16) 2010; 5 Raman, Raguram, Venkataraman, Paulson, Sasisekharan (CR1) 2005; 2 Lever, Page (CR11) 2002; 1 Guimond, Turnbull (CR12) 2004; 16 Vogt (CR10) 2003; 101 CR6 Rabenstein (CR5) 2002; 19 Volpi, Linhardt (CR17) 2010; 5 CR23 Skidmore (CR25) 2006; 1135 Scholefield (CR8) 2003; 163 Linhardt, Turnbull, Wang, Loganathan, Gallagher (CR19) 1990; 29 Kinoshita, Sugahara (CR27) 1999; 269 Guimond (CR18) 2009; 284 Ford-Perriss (CR15) 2002; 12 KJ Drummond (BFnprot2010145_CR22) 2001; 1 R Raman (BFnprot2010145_CR1) 2005; 2 H Toyoda (BFnprot2010145_CR26) 1999; 830 Y Huang (BFnprot2010145_CR24) 1996; 240 AM Vogt (BFnprot2010145_CR10) 2003; 101 MA Skidmore (BFnprot2010145_CR9) 2008; 51 SE Guimond (BFnprot2010145_CR14) 1999; 9 JE Turnbull (BFnprot2010145_CR20) 1999; 96 M Ford-Perriss (BFnprot2010145_CR15) 2002; 12 MA Skidmore (BFnprot2010145_CR29) 2009; 534 JE Turnbull (BFnprot2010145_CR7) 1991; 273 SE Guimond (BFnprot2010145_CR18) 2009; 284 BFnprot2010145_CR6 A Kinoshita (BFnprot2010145_CR27) 1999; 269 JA Deakin (BFnprot2010145_CR21) 2008; 18 M Ambrosius (BFnprot2010145_CR28) 2008; 1201 DL Rabenstein (BFnprot2010145_CR5) 2002; 19 R Lever (BFnprot2010145_CR11) 2002; 1 Z Scholefield (BFnprot2010145_CR8) 2003; 163 AK Powell (BFnprot2010145_CR16) 2010; 5 JE Turnbull (BFnprot2010145_CR2) 2007; 3 S Guimond (BFnprot2010145_CR12) 2004; 16 RJ Linhardt (BFnprot2010145_CR19) 1990; 29 J Turnbull (BFnprot2010145_CR4) 2001; 11 AK Powell (BFnprot2010145_CR13) 2004; 14 N Volpi (BFnprot2010145_CR17) 2010; 5 BFnprot2010145_CR23 MA Skidmore (BFnprot2010145_CR25) 2006; 1135 JR Bishop (BFnprot2010145_CR3) 2007; 446
References_xml	– volume: 101 start-page: 2405 year: 2003 end-page: 2411 ident: CR10 article-title: Heparan sulfate on endothelial cells mediates the binding of -infected erythrocytes via the DBL1alpha domain of PfEMP1 publication-title: Blood doi: 10.1182/blood-2002-07-2016 – volume: 273 start-page: 553 issue: Part 3 year: 1991 end-page: 559 ident: CR7 article-title: Distribution of iduronate 2-sulphate residues in heparan sulphate. Evidence for an ordered polymeric structure publication-title: Biochem. J. doi: 10.1042/bj2730553 – volume: 11 start-page: 75 year: 2001 end-page: 82 ident: CR4 article-title: Heparan sulfate: decoding a dynamic multifunctional cell regulator publication-title: Trends Cell Biol. doi: 10.1016/S0962-8924(00)01897-3 – volume: 2 start-page: 817 year: 2005 end-page: 824 ident: CR1 article-title: Glycomics: an integrated systems approach to structure-function relationships of glycans publication-title: Nat. Methods doi: 10.1038/nmeth807 – volume: 29 start-page: 2611 year: 1990 end-page: 2617 ident: CR19 article-title: Examination of the substrate-specificity of heparin and heparan-sulfate lyases publication-title: Biochemistry doi: 10.1021/bi00462a026 – volume: 1 start-page: 140 year: 2002 end-page: 148 ident: CR11 article-title: Novel drug development opportunities for heparin publication-title: Nat. Rev. Drug Discov. doi: 10.1038/nrd724 – volume: 240 start-page: 227 year: 1996 end-page: 234 ident: CR24 article-title: Simultaneous determination of dermatan sulfate and oversulfated dermatan sulfate in plasma by high-performance liquid chromatography with postcolumn fluorescence derivatization publication-title: Anal. Biochem. doi: 10.1006/abio.1996.0352 – volume: 16 start-page: 159 year: 2004 end-page: 160 ident: CR12 article-title: Proteoglycans make the grade-ient publication-title: Mol. Cell doi: 10.1016/j.molcel.2004.10.008 – volume: 9 start-page: 1343 year: 1999 end-page: 1346 ident: CR14 article-title: Fibroblast growth factor receptor signalling is dictated by specific heparan sulphate saccharides publication-title: Curr. Biol. doi: 10.1016/S0960-9822(00)80060-3 – ident: CR6 – volume: 830 start-page: 197 year: 1999 end-page: 201 ident: CR26 article-title: Rapid and sensitive analysis of disaccharide composition in heparin and heparan sulfate by reversed-phase ion-pair chromatography on a 2 μm porous silica gel column publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(98)00854-1 – volume: 14 start-page: 17R year: 2004 end-page: 30R ident: CR13 article-title: Interactions of heparin/heparan sulfate with proteins: appraisal of structural factors and experimental approaches publication-title: Glycobiology doi: 10.1093/glycob/cwh051 – ident: CR23 – volume: 269 start-page: 367 year: 1999 end-page: 378 ident: CR27 article-title: Microanalysis of glycosaminoglycan-derived oligosaccharides labeled with a fluorophore 2-aminobenzamide by high-performance liquid chromatography: application to disaccharide composition analysis and exosequencing of oligosaccharides publication-title: Anal. Biochem. doi: 10.1006/abio.1999.4027 – volume: 163 start-page: 97 year: 2003 end-page: 107 ident: CR8 article-title: Heparan sulfate regulates amyloid precursor protein processing by BACE1, the Alzheimer's beta-secretase publication-title: J. Cell Biol. doi: 10.1083/jcb.200303059 – volume: 1 start-page: 304 year: 2001 end-page: 310 ident: CR22 article-title: Electrophoretic sequencing of heparin/heparan sulfate oligosaccharides using a highly sensitive fluorescent end label publication-title: Proteomics doi: 10.1002/1615-9861(200102)1:2<304::AID-PROT304>3.0.CO;2-B – volume: 284 start-page: 25714 year: 2009 end-page: 25722 ident: CR18 article-title: Rapid purification and high sensitivity analysis of heparan sulfate from cells and tissues: toward glycomics profiling publication-title: J. Biol. Chem. doi: 10.1074/jbc.M109.032755 – volume: 446 start-page: 1030 year: 2007 end-page: 1037 ident: CR3 article-title: Heparan sulphate proteoglycans fine-tune mammalian physiology publication-title: Nature doi: 10.1038/nature05817 – volume: 96 start-page: 2698 year: 1999 end-page: 2703 ident: CR20 article-title: A strategy for rapid sequencing of heparan sulfate and heparin saccharides publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.96.6.2698 – volume: 5 start-page: 821 year: 2010 end-page: 833 ident: CR16 article-title: Generating heparan sulfate saccharide libraries for glycomics applications publication-title: Nat. Protoc. doi: 10.1038/nprot.2010.17 – volume: 5 start-page: 993 year: 2010 end-page: 1004 ident: CR17 article-title: High-performance liquid chromatography-mass spectrometry for mapping and sequencing glycosaminoglycan-derived oligosaccharides publication-title: Nat. Protoc. doi: 10.1038/nprot.2010.48 – volume: 19 start-page: 312 year: 2002 end-page: 331 ident: CR5 article-title: Heparin and heparan sulfate: structure and function publication-title: Nat. Prod. Rep. doi: 10.1039/b100916h – volume: 51 start-page: 1453 year: 2008 end-page: 1458 ident: CR9 article-title: Disruption of rosetting in malaria with chemically modified heparin and low molecular weight derivatives possessing reduced anticoagulant and other serine protease inhibition activities publication-title: J. Med. Chem. doi: 10.1021/jm701337t – volume: 18 start-page: 483 year: 2008 end-page: 491 ident: CR21 article-title: A simplified and sensitive fluorescent method for disaccharide analysis of both heparan sulfate and chondroitin/dermatan sulfates from biological samples publication-title: Glycobiology doi: 10.1093/glycob/cwn028 – volume: 1135 start-page: 52 year: 2006 end-page: 56 ident: CR25 article-title: High sensitivity separation and detection of heparan sulfate disaccharides publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2006.09.064 – volume: 3 start-page: 74 year: 2007 end-page: 77 ident: CR2 article-title: Emerging glycomics technologies publication-title: Nat. Chem. Biol. doi: 10.1038/nchembio0207-74 – volume: 12 start-page: 721 year: 2002 end-page: 727 ident: CR15 article-title: Variant heparan sulfates synthesized in developing mouse brain differentially regulate FGF signaling publication-title: Glycobiology doi: 10.1093/glycob/cwf072 – volume: 1201 start-page: 54 year: 2008 end-page: 60 ident: CR28 article-title: Quantitative determination of the glycosaminoglycan Delta-disaccharide composition of serum, platelets and granulocytes by reversed-phase high-performance liquid chromatography publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2008.06.007 – volume: 534 start-page: 157 year: 2009 end-page: 169 ident: CR29 article-title: Labelling heparan sulphate saccharides with chromophore, fluorescence and mass tags for HPLC and MS separations publication-title: Methods Mol. Biol. – volume: 3 start-page: 74 year: 2007 ident: BFnprot2010145_CR2 publication-title: Nat. Chem. Biol. doi: 10.1038/nchembio0207-74 – volume: 101 start-page: 2405 year: 2003 ident: BFnprot2010145_CR10 publication-title: Blood doi: 10.1182/blood-2002-07-2016 – volume: 1135 start-page: 52 year: 2006 ident: BFnprot2010145_CR25 publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2006.09.064 – volume: 19 start-page: 312 year: 2002 ident: BFnprot2010145_CR5 publication-title: Nat. Prod. Rep. doi: 10.1039/b100916h – volume: 830 start-page: 197 year: 1999 ident: BFnprot2010145_CR26 publication-title: J. Chromatogr. A doi: 10.1016/S0021-9673(98)00854-1 – ident: BFnprot2010145_CR6 doi: 10.1016/B978-008044859-6/50007-1 – volume: 14 start-page: 17R year: 2004 ident: BFnprot2010145_CR13 publication-title: Glycobiology doi: 10.1093/glycob/cwh051 – volume: 1 start-page: 140 year: 2002 ident: BFnprot2010145_CR11 publication-title: Nat. Rev. Drug Discov. doi: 10.1038/nrd724 – volume: 18 start-page: 483 year: 2008 ident: BFnprot2010145_CR21 publication-title: Glycobiology doi: 10.1093/glycob/cwn028 – volume: 16 start-page: 159 year: 2004 ident: BFnprot2010145_CR12 publication-title: Mol. Cell doi: 10.1016/j.molcel.2004.10.008 – volume: 96 start-page: 2698 year: 1999 ident: BFnprot2010145_CR20 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.96.6.2698 – volume: 5 start-page: 993 year: 2010 ident: BFnprot2010145_CR17 publication-title: Nat. Protoc. doi: 10.1038/nprot.2010.48 – volume: 1201 start-page: 54 year: 2008 ident: BFnprot2010145_CR28 publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2008.06.007 – volume: 273 start-page: 553 issue: Part 3 year: 1991 ident: BFnprot2010145_CR7 publication-title: Biochem. J. doi: 10.1042/bj2730553 – volume: 12 start-page: 721 year: 2002 ident: BFnprot2010145_CR15 publication-title: Glycobiology doi: 10.1093/glycob/cwf072 – volume: 284 start-page: 25714 year: 2009 ident: BFnprot2010145_CR18 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M109.032755 – volume: 269 start-page: 367 year: 1999 ident: BFnprot2010145_CR27 publication-title: Anal. Biochem. doi: 10.1006/abio.1999.4027 – volume: 5 start-page: 821 year: 2010 ident: BFnprot2010145_CR16 publication-title: Nat. Protoc. doi: 10.1038/nprot.2010.17 – volume: 240 start-page: 227 year: 1996 ident: BFnprot2010145_CR24 publication-title: Anal. Biochem. doi: 10.1006/abio.1996.0352 – volume: 51 start-page: 1453 year: 2008 ident: BFnprot2010145_CR9 publication-title: J. Med. Chem. doi: 10.1021/jm701337t – volume: 534 start-page: 157 year: 2009 ident: BFnprot2010145_CR29 publication-title: Methods Mol. Biol. – volume: 163 start-page: 97 year: 2003 ident: BFnprot2010145_CR8 publication-title: J. Cell Biol. doi: 10.1083/jcb.200303059 – volume: 446 start-page: 1030 year: 2007 ident: BFnprot2010145_CR3 publication-title: Nature doi: 10.1038/nature05817 – volume: 11 start-page: 75 year: 2001 ident: BFnprot2010145_CR4 publication-title: Trends Cell Biol. doi: 10.1016/S0962-8924(00)01897-3 – volume: 29 start-page: 2611 year: 1990 ident: BFnprot2010145_CR19 publication-title: Biochemistry doi: 10.1021/bi00462a026 – volume: 1 start-page: 304 year: 2001 ident: BFnprot2010145_CR22 publication-title: Proteomics doi: 10.1002/1615-9861(200102)1:2<304::AID-PROT304>3.0.CO;2-B – volume: 2 start-page: 817 year: 2005 ident: BFnprot2010145_CR1 publication-title: Nat. Methods doi: 10.1038/nmeth807 – ident: BFnprot2010145_CR23 – volume: 9 start-page: 1343 year: 1999 ident: BFnprot2010145_CR14 publication-title: Curr. Biol. doi: 10.1016/S0960-9822(00)80060-3
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Snippet	One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an...
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SubjectTerms	631/1647/2230/1378 631/1647/666/2260 631/45/72/1205 Analytical Chemistry Anticoagulants Biological Techniques Biomedical and Life Sciences Boron Compounds Carbohydrates Chromatography, High Pressure Liquid - methods Composition Computational Biology/Bioinformatics Disaccharides - isolation & purification Enzymes Fluorescence Fluorescent Dyes Heparan sulfate Heparin Heparin - analysis Heparitin Sulfate - analysis High performance liquid chromatography Life Sciences Liquid chromatography Methods Microarrays Organic Chemistry Properties protocol Saccharides Spectrophotometry, Ultraviolet - methods Sugars Sulfates
Title	Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection
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