Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Str...

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Published in:Scientific reports Vol. 12; no. 1; pp. 17785 - 6
Main Authors: Ongenae, Véronique, Azeredo, Joana, Kropinski, Andrew M., Rozen, Daniel, Briegel, Ariane, Claessen, Dennis
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 22.10.2022
Nature Publishing Group
Nature Portfolio
Subjects:
631/326
631/326/325
631/326/432
Bacteria
Bacteriophages - genetics
Biology
DNA, Viral - genetics
Genomes
Humanities and Social Sciences
Infectivity
Integrase
Integrases
Microscopy
Morphology
multidisciplinary
New species
Nucleotide sequence
pH effects
Phages
Phylogenetics
Proteins
Science
Science (multidisciplinary)
Serine
Soil
Soil microorganisms
Streptomyces
Streptomyces - genetics
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora . Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25–45 °C) and at pH ≥ 7.0, but lost infectivity at temperatures above 55 °C or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus , within the subfamily Arquattrovirinae .
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-22784-y