A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells

Isogenic mutants of Porphyromonas gingivalis which differ in the expression of fimbriae were used to examine the contribution of fimbriae in invasion of a human oral epithelial cell line (KB). At a multiplicity of infection of 100, the wild-type P. gingivalis strains 33277, 381, and A7436 exhibited...

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Veröffentlicht in:Infection and immunity Jg. 65; H. 5; S. 1980
Hauptverfasser: Njoroge, T, Genco, R J, Sojar, H T, Hamada, N, Genco, C A
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.05.1997
Schlagworte:
Antibodies, Blocking
Bacterial Adhesion - genetics
Bacterial Proteins - genetics
Bacteroidaceae Infections - genetics
Bacteroidaceae Infections - microbiology
Fimbriae Proteins
Fimbriae, Bacterial - genetics
Fimbriae, Bacterial - immunology
Fimbriae, Bacterial - physiology
Humans
KB Cells - ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Mouth Mucosa - microbiology
Porphyromonas gingivalis - genetics
Porphyromonas gingivalis - pathogenicity
Porphyromonas gingivalis - ultrastructure
ISSN:0019-9567
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Zusammenfassung:Isogenic mutants of Porphyromonas gingivalis which differ in the expression of fimbriae were used to examine the contribution of fimbriae in invasion of a human oral epithelial cell line (KB). At a multiplicity of infection of 100, the wild-type P. gingivalis strains 33277, 381, and A7436 exhibited adherence efficiencies of 5.5, 0.11, and 5.0%, respectively, and invasion efficiencies of 0.15, 0.03, and 0.10%, respectively. However, adherence to and invasion of KB cells was not detected with the P. gingivalis fimA mutants, DPG3 and MPG1. Adherence of P. gingivalis wild-type strains to KB cells was completely inhibited by the addition of hyperimmune sera raised to the major fimbriae. Examination by electron microscopy of invasion of epithelial cells by the P. gingivalis wild-type strain 381 revealed microvillus-like extensions around adherent bacteria; this was not observed with P. gingivalis fim mutants. Taken together, these results indicate that the P. gingivalis major fimbriae are required for adherence to and invasion of oral epithelial cells.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0019-9567
DOI:10.1128/iai.65.5.1980-1984.1997