Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms

Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly unde...

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Vydáno v:	The ISME Journal Ročník 14; číslo 6; s. 1369 - 1383
Hlavní autoři:	Avcı, Burak, Krüger, Karen, Fuchs, Bernhard M., Teeling, Hanno, Amann, Rudolf I.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.06.2020 Oxford University Press Nature Publishing Group
Témata:	14/19 14/32 38/39 45/23 45/43 631/158/855 631/326/41/2142 631/326/41/2535 Algae Algal blooms Bacteria BASIC BIOLOGICAL SCIENCES Biodegradation Biomedical and Life Sciences Chlorophyta - growth & development Chlorophyta - metabolism Chlorophyta - microbiology Community composition Degradation Ecology Emergency response Eutrophication Evolutionary Biology First responders Flavobacteriaceae - classification Flavobacteriaceae - genetics Flavobacteriaceae - isolation & purification Flavobacteriaceae - metabolism Fluorescence Fluorescence in situ hybridization Gene expression Genomes Glycan Heterotrophic bacteria In Situ Hybridization, Fluorescence Life Sciences marine microbiology Metagenome metagenomics Microbial Ecology Microbial Genetics and Genomics Microbiology Microorganisms Niche overlap North Sea Phylogeny Polysaccharides Polysaccharides - metabolism Saccharides Seasons Substrates Sympatric populations Xylan North Sea
ISSN:	1751-7362, 1751-7370, 1751-7370
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Shrnutí:	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood. We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp. (class Flavobacteriia ; known to include relevant polysaccharide-degraders) that were abundant during 2009–2012 spring algal blooms in the southern North Sea. We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics. Four clades with distinct polysaccharide niches were dominating. Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities. Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential. Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan. Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms. These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 German Research Foundation (DFG) AC02-05CH11231; AM 73/9-1; TE 813/2-1; FU 627/2-1 USDOE Office of Science (SC)
ISSN:	1751-7362 1751-7370 1751-7370
DOI:	10.1038/s41396-020-0601-y