Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms

Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly unde...

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Bibliographic Details
Published in:The ISME Journal Vol. 14; no. 6; pp. 1369 - 1383
Main Authors: Avcı, Burak, Krüger, Karen, Fuchs, Bernhard M., Teeling, Hanno, Amann, Rudolf I.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.06.2020
Oxford University Press
Nature Publishing Group
Subjects:
14/19
14/32
38/39
45/23
45/43
631/158/855
631/326/41/2142
631/326/41/2535
Algae
Algal blooms
Bacteria
BASIC BIOLOGICAL SCIENCES
Biodegradation
Biomedical and Life Sciences
Chlorophyta - growth & development
Chlorophyta - metabolism
Chlorophyta - microbiology
Community composition
Degradation
Ecology
Emergency response
Eutrophication
Evolutionary Biology
First responders
Flavobacteriaceae - classification
Flavobacteriaceae - genetics
Flavobacteriaceae - isolation & purification
Flavobacteriaceae - metabolism
Fluorescence
Fluorescence in situ hybridization
Gene expression
Genomes
Glycan
Heterotrophic bacteria
In Situ Hybridization, Fluorescence
Life Sciences
marine microbiology
Metagenome
metagenomics
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microorganisms
Niche overlap
North Sea
Phylogeny
Polysaccharides
Polysaccharides - metabolism
Saccharides
Seasons
Substrates
Sympatric populations
Xylan
North Sea
ISSN:1751-7362, 1751-7370, 1751-7370
Online Access:Get full text
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Summary:Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood. We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp. (class Flavobacteriia ; known to include relevant polysaccharide-degraders) that were abundant during 2009–2012 spring algal blooms in the southern North Sea. We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics. Four clades with distinct polysaccharide niches were dominating. Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities. Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential. Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan. Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms. These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.
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German Research Foundation (DFG)
AC02-05CH11231; AM 73/9-1; TE 813/2-1; FU 627/2-1
USDOE Office of Science (SC)
ISSN:1751-7362
1751-7370
1751-7370
DOI:10.1038/s41396-020-0601-y