Synthesis and Antineoplastic Activity of a Dimer, Spiroindolinone Pyrrolidinecarboxamide

The mutation or function loss of tumour suppressor p53 plays an important role in abnormal cell proliferation and cancer generation. Murine Double Minute 2 (MDM2) is one of the key negative regulators of p53. p53 reactivation by inhibiting MDM2–p53 interaction represents a promising therapeutic opti...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Molecules (Basel, Switzerland) Ročník 28; číslo 9; s. 3912
Hlavní autori: Cui, Jingyi, Wang, Yujie, Li, Xiaoxin, Xiao, Fei, Ren, Hongjun, Wu, Meng
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 05.05.2023
MDPI
Predmet:
Animals
Antineoplastic Agents - pharmacology
Apoptosis
Cancer
cancer treatment
Care and treatment
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation
dimer spiroindolinone pyrrolidinecarboxamide
Genes
Genetic aspects
Health aspects
Humans
Hydrochloric acid
Influence
MDM2 inhibitor
Mice
Mutation
p53 activation
Prevention
Prostate cancer
Proteins
Proteolysis
Proto-Oncogene Proteins c-mdm2 - metabolism
Regulatory approval
Tumor proteins
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Tumorigenesis
China
cancer treatment
dimer spiroindolinone pyrrolidinecarboxamide
p53 activation
MDM2 inhibitor
ISSN:1420-3049, 1420-3049
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:The mutation or function loss of tumour suppressor p53 plays an important role in abnormal cell proliferation and cancer generation. Murine Double Minute 2 (MDM2) is one of the key negative regulators of p53. p53 reactivation by inhibiting MDM2–p53 interaction represents a promising therapeutic option in cancer treatment. Here, to develop more effective MDM2 inhibitors with lower off-target toxicities, we synthesized a dimer, spiroindolinone pyrrolidinecarboxamide XR-4, with potent MDM2-p53 inhibition activity. Western blotting and qRT-PCR were performed to detect the impact of XR-4 on MDM2 and p53 protein levels and p53 downstream target gene levels in different cancers. Cancer cell proliferation inhibition and clonogenic activity were also investigated via the CCK8 assay and colony formation assay. A subcutaneous 22Rv1-derived xenografts mice model was used to investigate the in vivo anti-tumour activity of XR-4. The results reveal that XR-4 can induce wild-type p53 accumulation in cancer cells, upregulate the levels of the p53 target genes p21 and PUMA levels, and then inhibit cancer cell proliferation and induce cell apoptosis. XR-4 can also act as a homo-PROTAC that induces MDM2 protein degradation. Meanwhile, the in vivo study results show that XR-4 possesses potent antitumour efficacy and a favourable safety property. In summary, XR-4 is an interesting spiroindolinone pyrrolidinecarboxamide-derivative dimer with effective p53 activation activity and a cancer inhibition ability.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28093912