Creation of a bacterial cell controlled by a chemically synthesized genome

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic ch...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) Vol. 329; no. 5987; p. 52
Main Authors: Gibson, Daniel G, Glass, John I, Lartigue, Carole, Noskov, Vladimir N, Chuang, Ray-Yuan, Algire, Mikkel A, Benders, Gwynedd A, Montague, Michael G, Ma, Li, Moodie, Monzia M, Merryman, Chuck, Vashee, Sanjay, Krishnakumar, Radha, Assad-Garcia, Nacyra, Andrews-Pfannkoch, Cynthia, Denisova, Evgeniya A, Young, Lei, Qi, Zhi-Qing, Segall-Shapiro, Thomas H, Calvey, Christopher H, Parmar, Prashanth P, Hutchison, 3rd, Clyde A, Smith, Hamilton O, Venter, J Craig
Format: Journal Article
Language:English
Published: United States 02.07.2010
Subjects:
Bacterial Proteins - analysis
Base Sequence
Bioengineering
Cloning, Molecular
DNA, Bacterial - chemical synthesis
DNA, Bacterial - genetics
Escherichia coli - genetics
Gene Deletion
Genes, Bacterial
Genetic Engineering
Genome, Bacterial
Molecular Sequence Data
Mycoplasma capricolum - genetics
Mycoplasma mycoides - genetics
Mycoplasma mycoides - growth & development
Mycoplasma mycoides - physiology
Mycoplasma mycoides - ultrastructure
Phenotype
Plasmids
Polymerase Chain Reaction
Polymorphism, Genetic
Saccharomyces cerevisiae - genetics
Transformation, Bacterial
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.1190719