Alterations in peripheral blood lymphocyte cytokine expression in obesity

Summary Obesity is characterized by alterations in immune and inflammatory function. In order to evaluate the potential role of cytokine expression by peripheral blood mononuclear cells (PBMC) in obesity‐associated inflammation, we studied serum protein levels and mRNA levels in PBMC of interleukin...

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Bibliographic Details
Published in:Clinical and experimental immunology Vol. 146; no. 1; pp. 39 - 46
Main Authors: O'Rourke, R. W., Kay, T., Lyle, E. A., Traxler, S. A., Deveney, C. W., Jobe, B. A., Roberts, C. T., Marks, D., Rosenbaum, J. T.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.10.2006
Blackwell
Oxford University Press
Blackwell Science Inc
Subjects:
Adult
Biological and medical sciences
Body Mass Index
Cells, Cultured
Clinical Studies
cytokine
Cytokines - biosynthesis
Cytokines - blood
Cytokines - genetics
Enzyme-Linked Immunosorbent Assay - methods
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
Humans
Immunopathology
interleukin‐1 receptor antagonist
interleukin‐1β
interleukin‐6
Leptin - pharmacology
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - immunology
lymphocyte
Male
Medical sciences
Middle Aged
obesity
Obesity, Morbid - immunology
Obesity, Morbid - physiopathology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - genetics
tumour necrosis factor‐α
Immunopathology
Obesity
tumour necrosis factor-a
Interleukin 1 receptor antagonist
Nutrition disorder
Cytokine
Interleukin 1β
Blood
interleukin-6
interleukin-1β
Interleukin 6
Immunology
Tumor necrosis factor
Lymphocyte
Nutritional status
interleukin-1 receptor antagonist
ISSN:0009-9104, 1365-2249
Online Access:Get full text
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Summary:Summary Obesity is characterized by alterations in immune and inflammatory function. In order to evaluate the potential role of cytokine expression by peripheral blood mononuclear cells (PBMC) in obesity‐associated inflammation, we studied serum protein levels and mRNA levels in PBMC of interleukin (IL)‐6, IL‐1β, tumour necrosis factor (TNF)‐α and IL‐1Ra in nine lean and 10 obese subjects. Serum IL‐1β was undetectable, IL‐1Ra serum levels were elevated, serum levels of TNF‐α were decreased and serum levels of IL‐6 were similar in obese subjects compared to lean subjects, while transcript levels of IL‐6, IL‐1β and TNF‐α, but not IL‐1Ra, were decreased in PBMC from obese subjects. PBMC from obese subjects did, however, up‐regulate cytokine expression in response to leptin. Thus, obesity‐associated changes in IL‐1Ra serum levels and IL‐6 mRNA levels were not correlated with changes in cognate mRNA and serum levels, respectively, while TNF‐α serum levels and PBMC mRNA levels were both decreased in obese patients. While immune alterations in obesity are manifest in peripheral blood lymphocytes, the general lack of correlation between altered serum levels and altered PBMC gene expression suggests that PBMC may not be the source of aberrant serum cytokine levels in obesity.
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ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2006.03186.x