Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function

Background MicroRNAs are paramount in post transcriptional gene regulation. We investigated platelet miRNAs in patients with CAD and examined potential associations with course of left ventricular ejection fraction (LVEF%). Materials and methods In a first cohort, 62 MiRNAs were measured in platelet...

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Veröffentlicht in:BMC cardiovascular disorders Jg. 23; H. 1; S. 348 - 11
Hauptverfasser: Goldschmied, Andreas, Drotleff, Bernhard, Winter, Stefan, Schaeffeler, Elke, Schwab, Matthias, Gawaz, Meinrad, Geisler, Tobias, Rath, Dominik
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London BioMed Central 12.07.2023
BioMed Central Ltd
Springer Nature B.V
BMC
Schlagworte:
Acute coronary syndromes
Analysis
Angiology
Atherosclerosis
Blood platelets
Blood Transfusion Medicine
Cardiac output
Cardiac Surgery
Cardiology
Cardiovascular disease
Care and treatment
Catheters
Coronary artery disease
Coronary heart disease
Coronary vessels
Datasets
Diagnosis
Flow cytometry
Gene expression
Gene regulation
Genetic aspects
Health aspects
Heart
Heart attacks
Heart diseases
Hypotheses
Hypothesis testing
Internal Medicine
Left ventricular ejection fraction
Mann-Whitney U test
Measurement
Medicine
Medicine & Public Health
MicroRNA
MicroRNAs
miRNA
MiRNA 103a-3p
MiRNA 155-5p
Myocardial infarction
Platelet microRNA
Platelets
Vein & artery diseases
Ventricle
Germany
United States > US
Netherlands
Platelet microRNA
Myocardial infarction
MiRNA 103a-3p
Coronary artery disease
Left ventricular ejection fraction
MiRNA 155-5p
ISSN:1471-2261, 1471-2261
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Zusammenfassung:Background MicroRNAs are paramount in post transcriptional gene regulation. We investigated platelet miRNAs in patients with CAD and examined potential associations with course of left ventricular ejection fraction (LVEF%). Materials and methods In a first cohort, 62 MiRNAs were measured in platelets of 100 patients suffering from CAD. Expression profiles of individuals with chronic coronary syndrome (CCS) and MI were compared (CCS n = 67, MI n = 33). Also, associations between miRNA profiles and change in left ventricular ejection fraction (LVEF%) were investigated. In a second cohort of patients suffering from CCS (n = 10), MI (n = 11) or no CAD (n = 13), we measured miRNA expression in platelets, platelet supernatant and serum. This was carried out before and after in vitro platelet activation with CRP. Results Platelet miRNAs 103a-3p and 155-5p demonstrated higher expression in patients with CCS then in individuals with MI. Furthermore, multiple miRNAs were significantly higher expressed in matched controls compared to MI patients. 8 miRNAs showed higher expression in patients with improving LVEF% after a 1-year follow-up. In our second cohort, we found higher concentrations of 6 miRNAs in the platelet supernatant of patients with CCS, MI and no CAD after in vitro platelet activation. Most of these miRNAs showed a higher abundance in serum of MI patients as compared to CCS. Conclusion Several miRNAs show higher expression in platelets of CCS compared to MI. After in vitro platelet activation, a release of multiple miRNAs out of the thrombocyte was observed. Furthermore, upregulation of serum miRNAs was found in MI patients when compared to CCS patients and individuals without CAD. Hence, platelets could present a source of upregulated circulating miRNAs in MI and additionally affect course of LVEF%.
Bibliographie:ObjectType-Article-1
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ISSN:1471-2261
1471-2261
DOI:10.1186/s12872-023-03362-0