On the osteogenic differentiation of dental pulp stem cells by a fabricated porous nano-hydroxyapatite substrate loaded with sodium fluoride

In the present study, nano-hydroxyapatite (n-HA) powder was extracted from carp bone waste to fabricate porous n-HA substrates by a molding and sintering process. Subsequently, the substrates were loaded with different amounts of sodium fluoride (NaF) through immersion in NaF suspensions for 10, 7.5...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:BMC oral health Ročník 24; číslo 1; s. 1218 - 16
Hlavní autori: Arab, Samaneh, Bahraminasab, Marjan, Asgharzade, Samira, Doostmohammadi, Ali, Zadeh, Zahra Khatib, Nooshabadi, Vajihe Taghdiri
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London BioMed Central 14.10.2024
BioMed Central Ltd
Springer Nature B.V
BMC
Predmet:
Animals
Biomedical materials
Bones
Cell differentiation
Cell Differentiation - drug effects
Cell Survival - drug effects
Cells, Cultured
Cytotoxicity
Dental pulp
Dental Pulp - cytology
Dental Pulp - drug effects
Dentistry
Durapatite - chemistry
Durapatite - pharmacology
Enzymes
Ethanol
Fluorides
Fourier transforms
Humans
Hydrogen-Ion Concentration
Hydroxyapatite
Immersion
Medicine
Methods
Nano-hydroxyapatite
Nanoparticles
Oral and Maxillofacial Surgery
Osteogenesis - drug effects
pH effects
Physiological aspects
Polyvinyl alcohol
Porosity
Scanning electron microscopy
Sintering
Skin & tissue grafts
Sodium
Sodium fluoride
Sodium Fluoride - pharmacology
Spectrum analysis
Stem cells
Stem Cells - drug effects
Surface Properties
Tissue engineering
Toxicity
Iran
Germany
Sodium fluoride
Nano-hydroxyapatite
Tissue engineering
Stem cells
Dental pulp
ISSN:1472-6831, 1472-6831
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:In the present study, nano-hydroxyapatite (n-HA) powder was extracted from carp bone waste to fabricate porous n-HA substrates by a molding and sintering process. Subsequently, the substrates were loaded with different amounts of sodium fluoride (NaF) through immersion in NaF suspensions for 10, 7.5, and 5 min. The NaF-loaded n-HA substrates were then examined for their structural and physical properties, chemical bonds, loading and release profile, pH changes, cytotoxicity, and osteogenic effect on dental pulp stem cells (DPSCs) at the level of RNA and protein expression. The results showed that the n-HA substrates were porous (> 40% porosity) and had rough surfaces. The NaF could be successfully loaded on the substrates, which was 6.43, 4.50, and 1.47 mg, respectively for n-HA substrates with immersion times of 10, 7.5, and 5 min in the NaF suspensions. It was observed that the NaF release rate was rather fast during the first 24 h in all groups (39.06%, 36.43%, and 39.57% for 10, 7.5, and 5 min, respectively), and decreased dramatically after that, indicating a slow detachment of NaF. Furthermore, the pH of the medium related to all materials was changed during the first 4 days of immersion (from 7.38 to pH of about 7.85, 7.84, 7.63, and 7.66 for C0, C5, C7.5, and C10, respectively). The pH of media associated with the C7.5, and C10 increased up to 4 days and remained relatively constant until day 14 (pH = 7.6). The results of the cytotoxicity assay rejected any toxicity of the fabricated NaF-loaded n-HA substrates on DPSCs, and the cells could adhere to their surfaces with enlarged morphology. The results showed no effect on the osteogenic differentiation at the protein level. Nevertheless, this effect was observed at the gene level.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1472-6831
1472-6831
DOI:10.1186/s12903-024-04987-z