Simultaneous detection of pH value and glucose concentrations for wound monitoring applications

Aging population and longer life expectancy are the main reasons for an increasing number of patients with wound problems. Although the interest in wound care increases continuously, wound management still remains a challenge mainly due to the higher occurrence of chronic wounds, which require inten...

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Veröffentlicht in:Biosensors & bioelectronics Jg. 87; S. 312 - 319
Hauptverfasser: Jankowska, D.A., Bannwarth, M.B., Schulenburg, C., Faccio, G., Maniura-Weber, K., Rossi, R.M., Scherer, L., Richter, M., Boesel, L.F.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier B.V 15.01.2017
Schlagworte:
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensor
biosensors
Chromium
coatings
Constants
Enzymes
Enzymes, Immobilized - chemistry
Equipment Design
Fluoresceins - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Functional hydrogels
Glucose
Glucose - analysis
glucose oxidase
Glucose Oxidase - chemistry
Glucose sensing
Healing
Horseradish Peroxidase - chemistry
Humans
hydrocolloids
Hydrogels - chemistry
Hydrogen-Ion Concentration
longevity
Metabolites
Monitoring
patients
peroxidase
pH sensing
polysaccharides
Wound Healing
Wound monitoring
pH sensing
Wound monitoring
Metabolites
Biosensor
Glucose sensing
Functional hydrogels
ISSN:0956-5663, 1873-4235
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Zusammenfassung:Aging population and longer life expectancy are the main reasons for an increasing number of patients with wound problems. Although the interest in wound care increases continuously, wound management still remains a challenge mainly due to the higher occurrence of chronic wounds, which require intensive care and constant monitoring. Here, we demonstrate a fluorescent sensing system to monitor the wound status and to distinguish between an autonomously healing and a chronic wound at an early stage. The system allows monitoring two of the most relevant fluctuating wound parameters during the healing process which are pH and glucose concentration. A fluorescent pH indicator dye, carboxynaphthofluorescein, and a metabolite-sensing enzymatic system, based on glucose oxidase and horseradish peroxidase, were immobilized on a biocompatible polysaccharide matrix to develop a functional hydrogel coating for wound monitoring. The changes in metabolite and enzyme concentration in artificial wound extract were converted into a fluorescent signal. •A concept of a smart wound pad for monitoring of wound healing process is proposed.•The sensors detect changes in pH and glucose concentration in wound serum.•The sensing molecules were immobilized in a biocompatible hydrogel matrix.•The smart wound pad provides both colorimetric and fluorescent responses.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2016.08.072