The L1 retrotransposition assay: A retrospective and toolkit

LINE1s (L1s) are a class of mammalian non-LTR (long terminal repeat) retroelements that make up nearly 20% of the human genome. Because of the difficulty of studying the mobilization of endogenous L1s, an exogenous cell culture retrotransposition assay has become integral to research in L1 biology....

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Published in:Methods (San Diego, Calif.) Vol. 49; no. 3; pp. 219 - 226
Main Authors: Rangwala, Sanjida H., Kazazian, Haig H.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.11.2009
Subjects:
Animals
Cell culture assays
Cell Culture Techniques
Cell Line
Chickens
Cricetinae
Genetic Techniques
Humans
LINE1 (L1)
Long Interspersed Nucleotide Elements - physiology
Mice
Models, Genetic
Mutagenesis, Insertional
Non-LTR retrotransposons
Rats
Retrotransposition
Transgenic mice
LINE1 (L1)
Cell culture assays
Retrotransposition
Non-LTR retrotransposons
Transgenic mice
ISSN:1046-2023, 1095-9130, 1095-9130
Online Access:Get full text
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Summary:LINE1s (L1s) are a class of mammalian non-LTR (long terminal repeat) retroelements that make up nearly 20% of the human genome. Because of the difficulty of studying the mobilization of endogenous L1s, an exogenous cell culture retrotransposition assay has become integral to research in L1 biology. This assay has allowed for investigation of the mechanism and consequences of mobilization of this retroelement, both in cell lines and in whole animal models. In this paper, we outline the genesis of in vitro retrotransposition systems which led to the development of the L1 retrotransposition assay in the mid-1990s. We then provide a retrospective, describing the many uses and variations of this assay, ending with caveats and predictions for future developments. Finally, we provide detailed protocols on the application of the retrotransposition assay, including lists of constructs available in the L1 research community and cell lines in which this assay has been applied.
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ISSN:1046-2023
1095-9130
1095-9130
DOI:10.1016/j.ymeth.2009.04.012