Thyroid hormones and their placental deiodination in normal and pre-eclamptic pregnancy

Pre-eclampsia is associated with lower serum selenium concentrations and glutathione peroxidase expression/activity; total thyroid hormones are also lower. We hypothesised that the placental selenoprotein deiodinase (D3) will be protected in pre-eclampsia due to the hierarchy of selenoprotein biosyn...

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Veröffentlicht in:Placenta (Eastbourne) Jg. 34; H. 5; S. 395 - 400
Hauptverfasser: Kurlak, L.O., Mistry, H.D., Kaptein, E., Visser, T.J., Broughton Pipkin, F.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Kidlington Elsevier Ltd 01.05.2013
Elsevier
Schlagworte:
Adult
Biological and medical sciences
Deiodinases
Embryology: invertebrates and vertebrates. Teratology
Female
Fetal Blood - chemistry
Fundamental and applied biological sciences. Psychology
Gene Expression
Gestational Age
Humans
Internal Medicine
Iodide Peroxidase - analysis
Iodide Peroxidase - genetics
Iodide Peroxidase - metabolism
Obstetrics and Gynecology
Placenta
Placenta - enzymology
Placenta - metabolism
Pre-eclampsia
Pre-Eclampsia - blood
Pre-Eclampsia - enzymology
Pregnancy
Pregnancy. Parturition. Lactation
RNA, Messenger - analysis
Selenium - blood
Thyroid hormones
Thyroid Hormones - metabolism
Thyrotropin - blood
Thyroxine - blood
Triiodothyronine - blood
Vertebrates: reproduction
Placenta
Pre-eclampsia
Thyroid hormones
Deiodinases
Pregnancy
Vertebrata
Mammalia
Pregnancy disorders
Fetal membrane
Preeclampsia
Thyroid hormone
Normal
Pregnancy toxemia
ISSN:0143-4004, 1532-3102, 1532-3102
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Zusammenfassung:Pre-eclampsia is associated with lower serum selenium concentrations and glutathione peroxidase expression/activity; total thyroid hormones are also lower. We hypothesised that the placental selenoprotein deiodinase (D3) will be protected in pre-eclampsia due to the hierarchy of selenoprotein biosynthesis in selenium deficiency. Venous blood and tissue from three standardised placental sites were obtained at delivery from 27 normotensive and 23 pre-eclamptic women. mRNA expression and enzyme activity were assessed for both deiodinases (D2 and D3); protein expression/localisation was also measured for D3. FT4, FT3 and TSH concentrations were measured in maternal and umbilical cord blood. No significant differences in D3 mRNA or protein expression between normotensive and pre-eclamptic pregnancies. There was a significant effect of sampling site on placental D3 activity only in pre-eclamptic women (P = 0.034; highest activity nearest the cord). A strong correlation between D3 mRNA expression and enzyme activity existed only in the pre-eclamptic group; further strengthened when controlling for maternal selenium (P < 0.002). No significant differences were observed between groups for any of the maternal thyroid hormones; umbilical TSH concentrations were significantly higher in the pre-eclamptic samples (P < 0.001). D3 mRNA and protein expression appear to be independent of selenium status. Nevertheless, the positive correlation between D3 mRNA expression and activity evident only in pre-eclampsia, suggests that in normotensive controls, where selenium is higher, translation is not affected, but in pre-eclampsia, where selenium is low, enzyme regulation may be altered. The raised umbilical TSH concentrations in pre-eclampsia may be an adaptive fetal response to maximise iodide uptake.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0143-4004
1532-3102
1532-3102
DOI:10.1016/j.placenta.2013.02.009