Reduced diversity in the early fecal microbiota of infants with atopic eczema

It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms. The purpose of the present study was to characterize the very early infantile microbiota by using a culture-ind...

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Vydáno v:	Journal of allergy and clinical immunology Ročník 121; číslo 1; s. 129 - 134
Hlavní autoři:	Wang, Mei, Karlsson, Caroline, Olsson, Crister, Adlerberth, Ingegerd, Wold, Agnes E., Strachan, David P., Martricardi, Paolo M., Åberg, Nils, Perkin, Michael R., Tripodi, Salvatore, Coates, Anthony R., Hesselmar, Bill, Saalman, Robert, Molin, Göran, Ahrné, Siv
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York, NY Mosby, Inc 2008 Elsevier Elsevier Limited
Témata:	Age Allergic diseases Allergies Allergy and Immunology Antibiotics Asthma Atopic eczema Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biological and medical sciences Deoxyribonucleic acid Dermatitis, Atopic - immunology Dermatitis, Atopic - microbiology diversity DNA DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Eczema Electrophoresis, Agar Gel Feces - microbiology Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic Variation Humans Hypotheses Immunologi inom det medicinska området Immunology in the Medical Area Immunopathology Infant Infant, Newborn intestinal microbiota Medical sciences Nutrition research Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Skin allergic diseases. Stinging insect allergies temporal temperature gradient gel electrophoresis terminal restriction fragment length polymorphism England Italy Sweden intestinal microbiota diversity T-RFLP TTGE temporal temperature gradient gel electrophoresis T-RF terminal restriction fragment length polymorphism Atopic eczema Cy5 Terminal restriction fragment Indodicarbocyanine Human Allergy Immunopathology Skin disease Gradient Temperature Digestive system Gel electrophoresis Gut Infant Atopy Immunology Restriction fragment length polymorphism Eczema Genetics Early Feces Molecular biology
ISSN:	0091-6749, 1097-6825, 1097-6825
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Shrnutí:	It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms. The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life. Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by means of terminal restriction fragment length polymorphism (T-RFLP) and temporal temperature gradient gel electrophoresis (TTGE) analysis of amplified 16S rRNA genes. By means of T-RFLP analysis, the median number of peaks, Shannon-Wiener index, and Simpson index of diversity were significantly less for infants with atopic eczema than for infants remaining healthy in the whole group and for the Swedish infants when AluI was used for digestion. The same was found when TTGE patterns were compared. In addition, TTGE analysis showed significantly less bands and lower diversity indices for the British atopic infants compared with those of the control subjects. There is a reduced diversity in the early fecal microbiota of infants with atopic eczema during the first 18 months of life.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0091-6749 1097-6825 1097-6825
DOI:	10.1016/j.jaci.2007.09.011