Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays

The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular Cytokine Staining (ICS) assays. The goal of the EQAPOL Flow Cytometry E...

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Veröffentlicht in:Journal of immunological methods Jg. 409; S. 44 - 53
Hauptverfasser: Staats, Janet S., Enzor, Jennifer H., Sanchez, Ana M., Rountree, Wes, Chan, Cliburn, Jaimes, Maria, Chan, Ray Chun-Fai, Gaur, Amitabh, Denny, Thomas N., Weinhold, Kent J.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.07.2014
Schlagworte:
Antigen specific
Biomarkers - analysis
Consensus
Cooperative Behavior
cytokines
Cytokines - analysis
Flow cytometry
Flow Cytometry - standards
Grading
Guideline Adherence - standards
HIV Infections - diagnosis
HIV Infections - immunology
HIV Infections - therapy
Human immunodeficiency virus
Humans
ICS
International Cooperation
Laboratories - standards
Laboratory Proficiency Testing - standards
monitoring
Monitoring, Immunologic - standards
Multicenter Studies as Topic - standards
Observer Variation
Practice Guidelines as Topic - standards
Predictive Value of Tests
Proficiency testing
Program Development
Program Evaluation
Quality Control
Quality Improvement
Quality Indicators, Health Care - standards
remediation
Reproducibility of Results
Site performance
Specimen Handling - standards
staining
ICS
Flow cytometry
Antigen specific
Grading
Site performance
Proficiency testing
ISSN:0022-1759, 1872-7905, 1872-7905
Online-Zugang:Volltext
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Zusammenfassung:The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular Cytokine Staining (ICS) assays. The goal of the EQAPOL Flow Cytometry External Quality Assurance Program (EQAP) is to provide proficiency testing and remediation for participating sites. The program is not punitive; rather, EQAPOL aims to help sites identify areas for improvement. EQAPOL utilizes a highly standardized ICS assay to minimize variability and readily identify those sites experiencing technical difficulties with their assays. Here, we report the results of External Proficiency 3 (EP3) where participating sites performed a 7-color ICS assay. On average, sites perform well in the Flow Cytometry EQAP (median score is “Good”). The most common technical issues identified by the program involve protocol adherence and data analysis; these areas have been the focus of site remediation. The EQAPOL Flow Cytometry team is now in the process of expanding the program to 8-color ICS assays. Evaluating polyfunctional ICS responses would align the program with assays currently being performed in support of HIV immune monitoring assays.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1759
1872-7905
1872-7905
DOI:10.1016/j.jim.2014.05.021