Identification and mode of inheritance of quantitative trait loci for secondary metabolite abundance in tomato

A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgressio...

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Vydané v:The Plant cell Ročník 27; číslo 3; s. 485 - 512
Hlavní autori: Alseekh, Saleh, Tohge, Takayuki, Wendenberg, Regina, Scossa, Federico, Omranian, Nooshin, Li, Jie, Kleessen, Sabrina, Giavalisco, Patrick, Pleban, Tzili, Mueller-Roeber, Bernd, Zamir, Dani, Nikoloski, Zoran, Fernie, Alisdair R
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 01.03.2015
Predmet:
Biosynthetic Pathways - genetics
Chromatography, Liquid
Chromosome Mapping
Chromosomes, Plant - genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Genetic Association Studies
Inbreeding
Inheritance Patterns - genetics
Mass Spectrometry
Metabolome - genetics
Metabolomics
Phenotype
Plant Proteins - genetics
Plant Proteins - metabolism
Polymorphism, Genetic
Quantitative Trait Loci - genetics
Reproducibility of Results
Secondary Metabolism - genetics
Solanum - genetics
Solanum - growth & development
Solanum - metabolism
Transcription Factors - metabolism
ISSN:1532-298X
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Shrnutí:A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1532-298X
DOI:10.1105/tpc.114.132266